摘要
为探究九香虫血淋巴对胃癌细胞SGC-7901体外增殖的抑制作用,本文以体外培养人胃癌细胞SGC-7901为材料,经九香虫血淋巴处理后,采用台盼蓝染色法检测细胞死亡率,并用倒置显微镜观察细胞形态学变化。结果表明:九香虫血淋巴能显著抑制胃癌细胞SGC-7901生长,且在浓度为5、10、20、30、40 mg/L时呈时间和剂量依赖性。
To unravel the inhibitory effects of Haemolymph on in vitro proliferation of human gastric carcinoma cell SGC-7901. the in vitro SGC-7901 cells were treated by Haemolymph from Aspongopus chinensis Dal-las, then the cellular mortality was examined by trypan blue staining, and the morphological changes of the cells were observed using inverted microscope. The results showed that haemolymph from A. chinensis could inhibit the in vitro proliferation of human gastric cell SGC-7901 with a dosage-and time-dependent effect within the concentration of 5, 10, 20, 30 and 40 mg/L.
出处
《山地农业生物学报》
2013年第2期119-122,共4页
Journal of Mountain Agriculture and Biology
基金
贵阳市科技局中药现代化项目"九香虫抗胃癌活性成分的分离提取与生物鉴定"(筑科合同[2012204]43号)
贵州大学研究生创新基金"九香虫抗胃癌活性组分的分离与纯化"(农科合同[2012018]号)
贵州大学SRT字[2011]044号
关键词
九香虫
血淋巴
胃癌
抑制作用
台盼蓝
Aspongopus chinensis Dallas
haemolymph
gastric carcinoma
inhibitory effects
Trypan blue
