摘要
目的严重脓毒症和脓毒症休克患者经常合并心肌功能障碍,而NO的产生可能是其重要原因。文中通过观察诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在脓毒症大鼠心肌细胞的表达,探讨辛伐他汀预处理对其表达的影响及其心肌保护作用。方法雌性Wistar大鼠45只随机分为3组(n=15):正常组(N组)、盲肠结扎穿孔组(CLP组)和辛伐他汀组(S组),辛伐他汀组喂养辛伐他汀20 mg/kg,1次/d,共2周。2周后辛伐他汀组与盲肠结扎穿孔组大鼠行盲肠结扎穿孔术。术后48 h取各组大鼠心肌组织制成石蜡切片,采用HE染色的方法观察组织病理学改变,采用免疫组化染色的方法观察心肌细胞iNOS的蛋白表达。结果 HE染色显示盲肠结扎穿孔组大鼠心肌纤维水肿、疏松、间质充血水肿,而辛伐他汀组心肌的组织病理学改变较盲肠结扎穿孔组轻。免疫组化染色显示盲肠结扎穿孔组大鼠心肌细胞iNOS蛋白表达于术后48h较正常组明显增加(P<0.05),辛伐他汀组大鼠心肌细胞iNOS蛋白表达较盲肠结扎穿孔组明显减弱(P<0.05),与正常组相比差异仍有统计学意义(P<0.05)。结论辛伐他汀可减弱脓毒症大鼠心肌细胞iNOS的蛋白表达。
Objective Myocardial dysfunction frequently accompanies severe sepsis and septic shock. The generation of ni- tric oxide（NO） may play a pivotal role in depression of myocytes in sepsis. We investigated the expressions of iNOS in cardiac myocytes of rats with sepsis and then discussed the effects Of simvastatin pretreatment on this process and heart protective effects of simvastatin. Methods Forty-five female Wistar rats were used in this study. The animals were randomly divided into 3 groups （n = 15 for each） ： normal group（ N）, cecal ligation and puncture group（ CLP）, and simvastatin group（S）. ①S group： simvastatin of 20 mg/kg was ad- ministread once a day for two weeks before operating.②S group and CLP group： the rats＇abdomen was opened, then the caecum was ligated and punctured. ③Samples of cardiac tissues of three groups were collected at 48 h after CLP, and all the samples were made to paraffin sections for HE staining and immunohistochemistry （IH） staining, which was applied to examine the expression of iNOS.Results HE staining： In the CLP group,myocardial fibers were edematous and curmbly, myocardial fiber mesenchyme was congestiveand edematous. In the S group, the histopathological changes were not as serious as that in CLP group. Immunohistochemistry staining： The expressions of iNOS in group CLP were significantly higher than that in group N at48h after CLP（P〈O, 05）. In group S, the expressionsof iNOS were weaker than that in group CLP （ P 〈 0.05 ）, but there was difference between group N and group S （ P 〈 0.05 ）. Conclusion Simvastatin could attenuate the expression of iNOS in cardiac myocytes of rats with sepsis, so it is thought that simvasta- tin may partially improve heart function in sepsis.
出处
《医学研究生学报》
CAS
北大核心
2013年第4期356-359,共4页
Journal of Medical Postgraduates
