摘要
背景:将地塞米松以某种涂层方式固定到体外循环管道表面,既可持续地起到抗炎作用,又不至于由于血药浓度过大而产生不良反应,最大限度地发挥地塞米松的抗炎作用。目的:研制新型抗凝血活性的地塞米松磷酸钠涂层管路,评价涂层管道稳定性、抗凝活性及抗血小板等性能。方法:用浓硫酸、聚乙烯亚胺依次预处理聚氯乙烯体外循环管路表面。分别通过预混、离子键两种方式制备地塞米松磷酸钠涂层聚氯乙烯体外循环管道,对涂层管道进行涂层物定量分析,以未涂层的聚氯乙烯体外循环管路为空白对照,评价3组抗凝血、抗血小板、抗蛋白黏附等性能及涂层聚氯乙烯体外循环管道的体外释放特性。结果与结论:预混及离子键制备的地塞米松磷酸钠涂层聚氯乙烯管道表面固定地塞米松磷酸钠的最大固定量分别为(2.06±0.68),(3.33±0.75)μg/cm2。预混制备的地塞米松磷酸钠涂层聚氯乙烯管道抗凝血、抗血小板及蛋白黏附效果优于离子键制备的地塞米松磷酸钠涂层聚氯乙烯管道和空白对照组(P<0.05),且体外释放效果优于离子键制备的地塞米松磷酸钠涂层聚氯乙烯管道。表明通过预混方式制备的地塞米松磷酸钠涂层缓释及抗凝性能良好,有抗血小板黏附及血栓形成的功能,能满足体外循环中短期转流的要求。
BACKGROUND： Dexamethasone as coating is fixed to the surface of cardiopulmonary bypass pipe, which can maintain sustainable anti-inflammatory effects and avoid adverse reactions due to excessive blood concentration, thereby maximizing the anti-inflammatory action of dexamethasone. OBJECTIVE： To develop a new coating method of dexamethasone sodium phosphate, which has anticoagulation activity, and to evaluate the performance of the coated pipeline, including the stability, anticoagulant and antiplatelet activity. METHODS： The surface of polyvinyl chloride pipelines in the extracorporeal circulation was successively pretreated with strong sulfuric acid and polyethylene imines. Dexamethasone sodium phosphate coated pipelines were made through two methods： ionic blond and premix. Then, a quantitative analysis was performed to evaluate anticoagulation, antiplatelet, resistance of protein adhesion and antithrombosis function. Non-coated polyvinyl chloride pipeline served as control group. RESULTS AND CONCLUSION： The biggest drug loadings were （2.06 ±0.68） and （3.33±0.75） μg/cm2 for dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix and ionic blond, respectively. In the anticoagulation, antiplatelet, and resistance of protein adhesion experiment, dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix were superior to those prepared by ionic blond and control group （P 〈 0.05）. Release in vitro experiment showed that dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix were also superior to those prepared by ionic blond. The findings indicate that the dexamethasone sodium phosphate coating prepared by premix shows better release and anticoagulation performance, as wel as forms antiplatelet adhesion and antithrombosis function, to meet the short-term extracorporeal circulation requirements.
出处
《中国组织工程研究》
CAS
CSCD
2013年第12期2177-2184,共8页
Chinese Journal of Tissue Engineering Research
基金
天津市科委支撑项目(11ZCGYSY02000)
课题名称:人工心肺支持系统肝素涂层管路研制后实验及临床应用研究~~
关键词
生物材料
生物材料与药物控释
地塞米松磷酸钠
海藻酸钠
聚氯乙烯
缓释
涂层
生物相容性
其他基金
生物材料图片文章
biomaterials
biomaterials and controlled drug release
dexamethasone sodium phosphate
sodiumalginate
polyvinyl chloride
slow-release
coating
biocompatibility
other grants-supported paper
biomaterialphotographs-containing paper
