摘要
目的观察地塞米松对体外培养大鼠胸主动脉平滑肌细胞(SMC)增殖的作用,探讨其对血管内支架介入术后远期再狭窄的防治价值。方法组织贴块法体外原代培养大鼠胸主动脉SMC,取第6～10代作实验用,MTF法检测地塞米松不同浓度和不同作用时间对SMC增殖的效果,并用RT-PCR法检测不同浓度地塞米松作用下大鼠胸主动脉SMC增殖细胞核抗原(PCNA)mRNA的表达情况。结果①地塞米松呈剂量依赖性抑制体外培养的大鼠SMC增殖,不同时间和不同浓度对其抑制程度不同。各浓度组之间总A值变化经方差分析差异有显著性,F=36．02,P＜0．001;两两比较较高浓度组10^(-6)及10^(-5) mol/L抑制率相似,A值无显著性差异,P=0．065;低浓度组10^(-11) mol/L与对照组相比差异无显著性,P=0．567;余各两两比较差异均有显著性,P＜0．001;②地塞米松以浓度依赖模式抑制大鼠SMC的PCNA mRNA表达。方差分析F= 15．407,P＜0．001。较低浓度组(10^(-9) mol/L及10^(-11)mol/L)与对照组相比,无显著性差异,P值分别为0．48及0．56。其余各组同对照组相比,差异有显著性,P＜0．05。结论地塞米松呈剂量依赖性抑制大鼠SMC增殖, 10^(-7) mol/L浓度以上可起效,并能持续抑制96 h以上,较低浓度地塞米松在作用时间延长时也有可能抑制SMC的增殖。
＂ Objective To evaluate the effect of dexamethaaone to the cultured rat thoracic aortic smooth muscle cells （SMC） in vitro, and explore the role on it＇s prevention and cure for the in-stent restenosis after vascular intervention. Methods The rat thoracic aortic SMC were harvested and cultured for six to ten passages. The cultured SMC were synchronized and then restimulated to enter the ceU cycle, and treated with incremental concentrations of dexamethaaone or without dexamethasone as control. The proliferative assay was performed with MTT method in the different time points after treatment. RT-PCR was performed to assay the level of proliferating ceU nuclear antigen （PCNA） mRNA. Results 1. Dexamethasone progressively inhibited rat aortic SMC proliferation in a fashion. The A value was statistically significant for different concentrations （F = 36.02, P 〈 0.001 ）. The effect was not significant for dexamethasone concentrations either between 10^-6 and 10^-5 mol/L （P = 0.065） or between 10^-11 mol/L and control group （P = 0.567）. 2. RT-PCR suggested dexamethasone significantly decreased rat aortic SMC PCNA mRNA transcription in a fashion. Statistical analysis indicated F = 15.407 and P 〈 0.001 by ANOVA. Comparing to the control, the corrected A value was not statistically significant at 10^-9 or 10^-11 mol/L groups by post hoc analysis. Conclusions Dexamethasone inhibits rat aortic SMC proliferation in a concentrationdependent fashion. The data suggest that effective action concentration is 10^-7 mol/L with persistent time up to 96 hours or more. Dexamethasone may play the inhibit role to SMC at lower concentration with prolonging action time.
出处
《介入放射学杂志》
CSCD
2007年第3期194-197,共4页
Journal of Interventional Radiology
关键词
地塞米松
血管平滑肌细胞
细胞增殖
Dexamethasone
Vascular smooth muscle cell
Cell proliferation
