摘要
肌内脂肪(intramuscular fat,IMF)是影响肉品质的重要因素,其实质是肌内脂肪细胞中的甘油三酯,而肌内脂肪细胞中的甘油三酯含量与脂肪代谢密切相关。该研究采用实时荧光定量PCR方法,检测不同H-FABP基因型滇南小耳猪肌内脂肪细胞中脂类合成代谢相关基因的表达,关联分析肌内脂肪细胞中脂类合成代谢基因的表达水平与甘油三酯含量的关系。研究结果表明:滇南小耳猪在HaeIII-RFLP、MspI-RFLP位点均无多态性,分别表现为DD、AA基因型;在HinfI-RFLP位点具有多态性,分别为HH、Hh、hh三种基因型。不同H-FABP基因型滇南小耳猪肌内脂肪细胞中甘油三酯的含量为:HH>Hh>hh。HH基因型的脂类合成代谢相关基因(H-FABP、A-FABP、SCD、ACC、FAS、DGAT-1)mRNA表达水平显著高于Hh、hh基因型(P<0.05)。肌内脂肪细胞中H-FABP、A-FABP、ACC、SCD、FAS、DGAT-1基因的mRNA表达水平与甘油三酯含量呈显著正相关(P<0.01)。总之,HH基因型滇南小耳猪肌内脂肪含量高可能是HH基因型脂类代谢相关基因的高表达水平引起的。肌内脂肪细胞脂类合成代谢相关基因mRNA表达水平与肌内脂肪细胞中甘油三酯含量呈显著正相关。
The intramuscular fat （IMF） is the main factor affecting meat quality traits. In fact, intramuscular fat is triglycerol （TG） depositing in intramuscular adipocytes. The contents of TG were decided by the lipid me- tabolism. This study analyzed the expression levels of lipid synthesis metabolism related genes in Diannan small- ear pigs with different H-FABP genotypes using Real-time PCR, and measured the correlation between the content of TG and the expression levels of genes. The results showed that Diannan small-ear pigs had no polymorphism at HaeIII-RFLP, MspI-RFLP location, and displayed genotypes of DD, AA, respectively. Diannan small-ear pigs had polymorphism at Hinfl-RFLP location, and displayed genotypes of HH, Hh, hh. The contents of TG in Dian- nan small-ear pigs with different H-FABP genotypes were HH〉Hh〉hh. The mRNA expression levels of lipogenic genes, including H-FABP, A-FABP, SCD, ACC, FAS and DGAT-1 were significantly higher in Hinfl PCR-RFLP HH genotypes than in other genotypes （P〈0.05）. The mRNA expression levels ofH-FABP, A-FABP, SCD, FAS, ACCand DGAT-1 genes and the content of TG in intramuscular adipocytes had significant positive correlation （P〈0.01）. In conclusion, the H-FABP genotypes affected the intramuscular fat content in Diannan small-ear pigs. The mRNA expression levels of lipogenic genes in intramuscular adipocytes and the content of TG in intramuscular adipocytes had si^nificantly positive correlation.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第5期661-667,共7页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31060331)
云南省自然科学基金(批准号:2009CD056)资助的课题~~
