摘要
以奶牛IL-8基因为研究对象,运用生物信息学方法,对其编码的蛋白质结构、理化性质、信号肽、跨膜结构、亚细胞定位、二级结构以及高级结构进行生物信息学分析,并推测与其他物种的进化关系。结果表明:奶牛IL-8蛋白为稳定亲水性跨膜蛋白,定位于细胞外。二级结构以α螺旋和无规则卷曲为主,有2个Ser和2个Thr,可能成为蛋白激酶磷酸化位点,此外还有12个糖基化位点。保守结构域分析表明,奶牛IL-8蛋白有一个SCY锌指保守结构域。IL-8蛋白氨基酸邻接系统树分析表明,奶牛IL-8基因与绵羊的亲缘关系最近,具有较高的同源性。
IL-8 gene in cattle was investigated. By means of bioinformatics method, mainly focused on analyzing its en- coding protein including its structure, physical and chemical properties, signal peptide, transmembrane structure, subcel- lular localization, secondary structure, higher structure and its evolution relationship with other species. The results indi- cated that cattle IL-8 protein was a stable hydrophilic transmembrane protein, located at cellular exterior. The secondary ,;tructure was primarily composed of a-helix and random coil. It had two serines and two threonines, which might become phosphorylation sites of the protein kinase. Besides, it had twelve glycosylation sites. The analysis of conservative struc- ture domain showed that cattle IL-8 protein had a SCY zinc finger conservative structure domain. The amino acid adjacen- cy phylogenetic tree analysis showed that cattle IL-8 gene had the closest genetic relationship and high homology with o- vines.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第1期18-22,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(31272407)
国家高技术研究发展计划项目(863-2008AA10Z144)
江苏省高校优势学科建设工程资助项目
