摘要
目的探讨有限稀释定量ＰＣＲ检测血清ＨＢＶ－ＤＮＡ对干扰素抗病毒治疗的指导意义。方法利用有限稀释定量ＰＣＲ技术分别检测 ３８例 ＨＢｓＡｇ、ＨＢｅＡｇ双阳性患者 α－２ｂ干扰素治疗前后血清 ＨＢＶ－ＤＮＡ含量。结果 ３８例患者干扰素治疗 １２周 ＨＢＶ－ＤＮＡ阴转７例（１８．４％）；治疗２４周阴转１１例（２８．９％）。其中病毒滴度＞２５０ｐｇ／ｍＬ者阴转１１．１％（１／９）；病毒滴度２５ｆｇ～２５０ｐｇ／ｍＬ者阴转２５．０％（４／１６）；病毒滴度＜２５ｆｇ／ｍＬ者阴转４６．２％（６／１３），三组比较Ｐ＜０．０１。结论有限稀释定量ＰＣＲ检测ＨＢＶ－ＤＮＡ对慢性乙型肝炎患者干扰素治疗具有一定的指导意义，以病毒滴度≤２５０ｐｇ／ｍＬ、干扰素治疗２４周疗效较佳。
To observe the directive value of limited dilution quantitative PCR determination of serum HBV-DNA on the efficacy of interferon anti-virus in patients with chronic hepatitis B. Method HBV-DNA concenhations were quantitatively determined before and after interferon treatment in 38 patients with HBsAg(+ ) and HBeAg(+ ) by the footed dilution PCR. Results Among 38 patients with interferon treatment for 12 and 24 weeks, HBV-DNA was negative in 7 cases (18 .4% ) and 11 cases (28. 9% ) respectively. There was only 1 case (11 .5% ) negative in the group of virus titer > 250 pg/ mL (9 cases), but 4 and 6 cases (25.0% and 46.2%) negative in the groupe of virus titer 25 fg~ 250 pg/mL and < 25fg/ mL (P < 0.01). Conclusions HBV-DNA concentration could be detendned quantitatively by limited dilution PCR, which showed some directive effect on interferon anti-virus in patients with chronic hepatitis B. The patients whose virus titer 250 pg/mL and treatment for 24 weeks with interferon would receive better effect.
出处
《胃肠病学和肝病学杂志》
CAS
2000年第3期184-185,共2页
Chinese Journal of Gastroenterology and Hepatology
