摘要
目的研究p53蛋白和增殖细胞核抗原（Ki一67）表达与阑尾黏液性肿瘤临床病理特征之间的关系以及二者在肿瘤发生、发展中的作用。方法选取解放军总医院病理科1993年5月至2007年10月收治的42例阑尾黏液性肿瘤患者手术标本组织蜡块，另取10例单纯性阑尾炎作为对照。应用PV6000免疫组织化学二步法，分别检测p53蛋白和Ki-67抗原的表达水平。结果p53蛋白在阑尾黏液性肿瘤中表达阳性率[31．0％（13／42）]高于阑尾炎组[0（0／10）]（Xz_4．127，P=0．042），在阑尾黏液腺癌组表达阳性率[40．0％（12／30）]高于低级别黏液性肿瘤组[8．3％（1／12）1（χ2=4．0218，P=0．044）。形成腹膜假黏液瘤组p53蛋白表达阳性率[45．5％（10／22）]高于无腹膜假黏液瘤组[15．0％（3／20）]（χ2=4．5464，P=0．033）。Ki．67抗原在阑尾黏液性肿瘤中标记阳性率[45．2％（19／42）]高于阑尾炎组[10．0％（1／10）1（χ2=4．2374，P=0．039），而Ki-67抗原的表达与性别、年龄、是否形成腹膜假黏液瘤以及肿瘤病理类型等因素均无关（χ2值分别为0．0961、1．5910、1．6155、2．7776，均P〉0．05）。阑尾黏液性肿瘤中，p53蛋白阳性表达组Ki-67标记阳性率高于p53蛋白阴性组（χ2=7．6299，P=0．0057）。结论p53基因的突变与阑尾黏液性肿瘤的发生、发展有一定关系。Ki-67抗原表达可以反映出阑尾黏液性肿瘤增殖活性，但单独检测Ki．67不能作为鉴别肿瘤良性及判定恶性程度的指标。p53基因突变与Ⅺ．67抗原表达存在相关性，联合检测p53蛋白、Ki-67抗原对于评估阑尾黏液性肿瘤的生物学行为，判断肿瘤恶性程度具有一定意义。
Objective To investigate the relationship between the expression of p53 protein and Ki-67 antigen in order to detect its clinical characters and the significance of them in them occurrence and development of appendiceal mucinous neoplasms. Methods 42 tissues of appendiceal mucinous neoplasms were selected by pathological diagnosis in the department of pathology of the PLA general hospital from May 1993 to October 2007. The 10 samples of control group were obtained from simple appendicitis after surgery. Applied PV6000 immunohistochemical method, the expressions of p53 protein and Ki-67 antigen were detected. Results The expression of p53 protein in appendiceal mucinous neoplasms was higher than that in appendicitis tissue [31.0 % （13/42） vs 0 （0/10）, χ2 = 4.127, P = 0.042）. The positive rate of p53 protein in appendiceal mucinous adenocarcinoma was higher than that in low-grade appendiceal mucinous neoplasm tissue （40.0 % vs 8.3 %, χ2 = 4.0218, P = 0.044）. The positive rate of p53 protein in appendiceal mucinous neoplasms tissue with pseudomyxoma peritonei was higher than that in tissue without PMP （45.5 % vs 15.0 %, χ2 = 4.5464, P = 0.033）. The expression of Ki-67 antigen in appendiceal mucinous neoplasms was higher than that in appendicitis tissue （45.2 % vs 10.0 %, χ2 = 4.2374, P = 0.039）. Considering the different factors （such as gender, age, pathological type and PMP）, Ki-67 antigen expressions were still not significantly different （P 〉 0.05）. In appendiceal mucinous neoplasms, the difference of positive rates of Ki-67 between the p53 positive expression group and the p53 negative group was statistically significant （χ2 = 7.6299, P = 0.0057）. Conclusion The expression of p53 protein was correlated with the expression of Ki-67 antigen. It＇s valuable that both p53 protein and Ki-67 antigen are used to evaluate the biological behavior of appendiceal mucinous neoplasms and predict the malignant degree.
出处
《肿瘤研究与临床》
CAS
2013年第4期234-237,共4页
Cancer Research and Clinic
