摘要
目的:研究消化性溃疡患者Toll样受体4的表达及血清TNF-α浓度水平,探讨其与幽门螺杆菌感染的关系。方法:选取24例幽门螺杆菌(helicobacter.pylori,HP)阳性和16例HP阴性消化性溃疡患者,分别运用半定量PCR(Semi-quantitative RT-PCR)法、酶联免疫吸附测定(enzyme-linked immuno sorbent assay,ELISA)法测定其外周血单个核细胞(peripheral blood mononuclearcells,PBMCs)Toll样受体4的表达及血清TNF-α浓度。结果:HP阳性和HP阴性消化性溃疡患者PBMCs中均有TLR-4 mRNA的表达,HP阳性患者PBMCs TLR-4 mRNA的表达显著高于HP阴性组患者(P<0.0l),HP阳性组患者血清TNF-α水平亦显著高于HP阴性组患者(P<0.0l),TLR-4 mRNA表达水平与血清TNF-α水平呈正相关(r=0.21,p=0.02)。结论:HP阳性消化性溃疡患者外周血单个核细胞TLR-4 mRNA表达水平上调,血清TNF-α水平也同步升高,TLR4与HP感染所致消化性溃疡病理过程密切相关。
Objective： To study the expression of toll like receptor 4 （TLR-4） mRNA on peripheral blood mononuclear cells （PBMCs） and tumor nectosis factor-α （TNF-α） levels in the serum of patients with peptic ulcer disease （PU）, to investigate the relation-ship among TLR-4, TNF-α and helicobacter. Pylori （HP）-associated peptic ulcer. Methods： The expression ofTLR-4 mRNA on PBMCs from 40 PU patients （24 HP positive and 16 HP negative ） were measured by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）, the serum levels of TNF-α were measured by enzyme-linked immuno sorbent assay （ELISA） in each group. Results： TLR-4mRNA expression were detectable in all the PU patients. The expression of TLR-4 on PBMCs in HP positive patients significantly increased compared to that in HP negative patients （P〈 0.01）, while the serum level of TNF-α in HP positive patients was significantly higher than that in HP negative patients （P〈 0,01）, there was a positive correlation between TLR-4 and TNF-α, Conclusion： The expres-sion of TLR-4mRNA on PBMCs of liP positive PU patients was elevated and correlated positively with the concentration of TNF-α, thus TLR-4 played an important role in the irnmmaopathogenesis of H.P-associated peptic ulcer.
出处
《现代生物医学进展》
CAS
2013年第6期1083-1085,共3页
Progress in Modern Biomedicine
