摘要
目的:建立以高效液相色谱法测定感冒止咳颗粒中葛根素含量的方法。方法:ZORBAX SB-C18(250mm×4.6mm,5μm),流动相为甲醇-水(28∶72),流速为1.0mL/min,检测波长为250nm,柱温为30℃。结果:葛根素在0.080 1～1.602 4μg/mL范围内呈良好的线性关系。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。
Objective：To establish a high performance liquid chromatography determination of puerarin in cold cough particle con tent of method. Methods：ZORBAX SB-C18 （250mm×4.6mm,5μm）, mobile phase was methanol and water （28 ：72）, flow rate is 1.0mL/min, detection wavelength was 250nm, column temperature was 30℃. Results： Puerarin of 0. 080 1 - 1. 602 4μg/mL .showed good linear relationship. Conclusion：This method is simple and accurate, good separating effect, wide linear range, high sensitivity, and can be used for the quality control of the product.
出处
《亚太传统医药》
2013年第5期52-54,共3页
Asia-Pacific Traditional Medicine
关键词
高效液相色谱法
感冒止咳颗粒
葛根素
含量
High Performance Liquid Chromatography
Cold Zhike Granules
Puerarin
Content
