摘要
目的:研究不同种源地广金钱草药材质量变异及遗传多样性,为合理利用广金钱草种质资源及优良品种选育奠定基础。方法:采用AFLP分子标记技术,对18个种源地广金钱草进行遗传多样性分析,用NTSYSpc-2.11F软件计算并分析广金钱草种质间的相似度,构建遗传系统进化树;采用HPLC测定药材夏佛塔苷含量,并进行方差分析。结果:通过筛选得到8对AFLP引物组合,共扩增出844个DNA条带,多态性条带为717个,多态性位点平均为84.27%;通过聚类分析,将18个种源地的广金钱草种质分为3大类。种源间广金钱草药材夏佛塔苷含量差异显著,海南三亚种源夏佛塔苷含量显著高于其他种源地药材夏佛塔苷含量。结论:不同种源广金钱草药材质量差异显著,遗传多样性丰富,蕴藏着优质的种质资源,可以进行综合开发及优良品种选育。
Objective： To explore the quality variation and genetic diversity of Desmodium styracifolium from different prove- nances, and lay a foundation for rational exploitation on germplasm resources and fine variety breeding of D. styracifolium. Method： Amplified fragment length polymorphism （AFLP） markers were developed to analyze genetic diversity in D. styracifolium from 18 re- sources. NTSYSpc-2.1 IF software was used to analyze the similarity among the D. styracifolium germplasms and construct the genetic phylogenetic tree. The schaftoside content in D. styracifolium from different provenances was determined by HPLC. Result： A total of 844 fragments were amplified with 8 primers, in which 717 were polymorphic bands, accounting for 84.27% of the total detected varia- tion. All the specimens from 18 resources could be grouped into 3 clusters by cluster analysis. The sehaftoside contents of D. styracifo- lium germplasms differed significantly, with the highest content in the germplasm from Sanya, Hainan. Conclusion： Significant quality variation and genetic diversity can be observed among D. styracifoliurn germplasms. The diverse germplasm resources should be ex- plored and the fine variety should be selected to breed.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第9期1344-1348,共5页
China Journal of Chinese Materia Medica
基金
国家科技基础性工作专项重点项目(2007FY110600)
中山市科技局华南现代中医药城项目(20101H020)
关键词
广金钱草
质量变异
遗传多样性
Desmodium styracifolium
quality variance
genetic diversity
