摘要
目的:研究不同种源高良姜的遗传多样性,探求各种质间的亲缘关系,为合理利用高良姜种质资源及优良品种选育奠定理论基础。方法:采用扩增片段长度多态性(AFLP)技术,对8个种源地的高良姜进行遗传多态性分析,将扩增出的条带作为原始矩阵,用NTSYSpc-2.11F软件计算并分析高良姜种质间的相似度,构建遗传系统进化树。结果:通过筛选得到8对AFLP引物组合,共扩增出1 120个DNA条带,多态性条带为1 044个,多态性位点平均为92.57%;通过构建系统进化树,将8个种源地的高良姜种质分为3类。结论:高良姜种质间存在较高多态性,遗传多样性丰富。
Objective： To explore the genetic diversity and relationship of different Alpinia officinarum germplasm. Method： Amplified fragment length polymorphism （AFLP） markers were developed to analyze genetic polymorphism in A. officinarum from eight resources. The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A. officinarum germplasm and to construct the genetic phylogenctic tree. Result： A total of 1 120 fragments were genotyped using AFLP with eight prime combinations. Analysis identified 1 044 polymorphic fragments, accounting for 92.57% of the total detected variation. Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A. officinarum. Conclu- sion： Significant polymorphism and genetic diversity can be observed among A. officinarum germplasm resources.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第3期330-333,共4页
China Journal of Chinese Materia Medica
基金
国家科技基础条件平台工作项目(2005DKA21000)
国家科技基础性工作专项重点项目(2007FY110600)
广东省科技计划项目(2009B060700076)
广东药学院博士启动项目
广东药学院重点培养青年教师项目
关键词
高良姜
种质资源
AFLP
遗传多样性
Alpinia officinarum
germplasm resources
amplified fragment length polymorphism （AFLP）
genetic diversity
