摘要
目的探讨HSVⅡ-DNA检测对生殖器疱疹中Ⅱ型单纯疱疹病毒(HSVⅡ)阳性检出率的应用价值。方法选取本院2010年7月～2012年7月性病门诊收治的生殖器疱疹患者135例为实验标本,分别予以荧光定量聚合酶链反应法(FQ-PCR)检测HSVⅡ-DNA和血清酶联免疫吸附法(ELISA)检测HSVⅡ抗体,再以病毒培养法评价以上两种方法的检出率。结果 FQ-PCR法HSVⅡ-DNA检测的HSVⅡ阳性检出率为74.1%,ELISA法HSVⅡ抗体检测的HSVⅡ阳性检出率为65.2%,作病毒培养标准检测的HSVⅡ阳性率为80.7%;FQ-PCR法检测HSVⅡ的灵敏度、阳性及阴性预测值、特异性显著高于ELISA法。结论 FQ-PCR法HSVⅡ-DNA检测的HSVⅡ阳性检出率更贴近于金标准值,并且检测上具有特异性强、灵敏度高、快速等优越性,对于临床诊断生殖器疱疹具有重要的应用价值,值得推广应用。
Objective To research on HSVⅡ -DNA detection of genital herpes （ HSV Ⅱ ） application value of positive detection rate. Methods Selected 135 cases of patients with genital herpes as experimental samples, were measured in 135 patients used FQ-PCR and ELISA methods, and training method in the evaluation of the above two methods to virus. Results FQ-PCR detection in patients with HSV positive rate was 74.1%, ELISA detection in patients with HSV positive rate was 65.2%, the positive rate of HSV for virus culture of detection was 80.7%; The sensitivity of FQ- PCR detection in patients with HSV, the positive and negative predictive value, the specificity was higher than that of ELISA method. Conclusion FQ-PCR detected in patients with HSV positive detection rate closer to the gold standard, and the detection has the advantages of high specificity, high sensitivity, fast advantages, is of great significance for the diagnosis of genital herpes, worthy of clinical application.
出处
《中国现代医生》
2013年第13期92-93,共2页
China Modern Doctor
关键词
阳性率
生殖器疱疹
病毒培养
单纯疱疹病毒
Positive rate of virus
Genital herpes
Culture
Herpes simplex virus
