摘要
旨在建立链脲菌素(Streptozotocin,STZ)诱导恒河猴糖尿病模型.选用健康成年恒河猴,实验分A、B、C三组,分别静脉给予50 mg/kg、100 mg/kg及150 mg/kg不同剂量STZ,检测成模前后猴血糖、血清C肽、糖化血红蛋白及血生化等指标,评价STZ诱导建立的猴糖尿病模型.结果显示造模成功率A组80%,B组100%,C组60%;各组糖尿病猴存活时间达12周以上,均出现体重下降;血糖和糖化血红蛋白水平术后1周开始上升,B组血糖水平高于A组(P<0.05),均高于造模前(P<0.05);口服糖耐量实验结果显示不同剂量STZ血糖水平在0～120 min内均呈上升趋势,处于较高水平,高于造模前(P<0.05);血清C肽对葡萄糖刺激不敏感,均小于0.5 ng/mL,明显低于与造模前(P<0.05);A组和B组肝肾功能指标均在正常范围,C组肝肾功能损伤严重,但各组对血液系统及血脂等指标无影响.因此100 mg/kg STZ可成功诱导非人类灵长类恒河猴糖尿模型,其成功率高,模型稳定,不影响肝肾功能.
The aim of this study is to establish a model of Streptozotocin （STZ） - induced diabetes in rhesus. Healthy adult rhesus are divided into three groups （n = 5） ： group A, B and C, who are injected at 50 mg/kg, 100 mg/kg and 150 mg/kg doses of streptozotocin, respectively. The blood glucose, serum C peptide, glycated hemoglobin and blood biochemistry are examined to evaluate this STZ - induced diabetes model. The success rate of the diabetes model is 80%, 100% and 60% in group A, B and C respectively. The survival time is more than 12 weeks, all with weight loss. The blood glucose and the glyeated hemoglobin levels in diabetic monkeys begin to rise after 1 week. The blood glucose levels are higher in group B than that in group A （ P 〈 0.05 ）, while both are higher than those before modeling （P 〈 0.05 ）. Oral glucose tolerance test results show that the blood glucose levels in diabetic monkeys are increased in 0 - 120 min, and maintain at a high level, higher than those before modeling （ P 〈 0.05 ）. Serum C peptide is insensitive to the stimulation of glucose, less than 0.5 ng/mL, Serum C peptide is insensitive to stimulation of glucose, less than 0.5ng/mL, and lower than that before modeling（P 〈 o. 05）. kidney proven The function of liver and kidne in group C is heavily damaged, that 100 mg/kg STZ can induce high success rate and stability, without y in group A and B are in normal range, while the function of liver and but there is no effect on the blood and blood lipid indexes. It is therefore successfully diabetes model in nonhuman primates rhesus, which has a affecting the function of liver and kidney.
出处
《昆明理工大学学报（自然科学版）》
CAS
北大核心
2013年第2期74-79,共6页
Journal of Kunming University of Science and Technology(Natural Science)
基金
云南省重大攻关项目(2009CA010)
云南省科技厅-昆医联合专项(2012FB101)
关键词
链脲菌素
恒河猴
C肽
血糖
糖耐量实验
streptozotocin
rhesus
C peptide
blood glucose
glucose tolerance test
