摘要
目的对HSV-1作抗原的玻片酶免疫法(EIA)用于B病毒抗体检测的可靠性进行研究。方法用HSV-1为抗原的玻片酶免疫法(EIA)和酶联免疫吸附法(ELISA)与B病毒为抗原的斑点酶免疫法(DIA)、免疫荧光法(IFA)及酶联免疫吸附法(ELISA)对猕猴血清进行B病毒抗体检测的比较分析。结果HSV1为抗原的EIA与B病毒为抗原的DIA、IFA和ELISA检测结果的符合率分别为100%、95.6%和97.4%;HSV1为抗原的EIA检测确定的阴性血清样本,经B病毒抗原的IFA检测均为阴性;在HSV1为抗原的EIA确定的779份阴性血清中,经B病毒抗原的ELISA检测,有2.6%(20份)为阳性;HSV-1抗原的EIA和HSV-1为抗原的ELISA检测均为阴性的20份血清样本,经B病毒抗原的ELISA检测,18份仍为检出阴性,2份为阳性。结论HSV-1为抗原的EIA的检测结果与B病毒抗原的DIA、IFA和ELISA的检测结果一致性较好。同时,用HSV-1抗原进行B病毒抗体检测,存在极少数阳性动物漏检的可能。
Objective To evaluate the reliability of enzyme immunoassay （EIA） of HSV-1 as antigen for detection of B virus antibodies. Methods B virus antibodies were detected with enzyme immunoassay （EIA, HSV-lantigen）, enzyme-linked immunosorbent assay （ELISA, HSV- 1 antigen）, immunobinding assay （ DIA , B virus antigen）, immunofluorecenee assay （IFA, B virus antigen） and enzyme-linked immunosorbent assay （ELISA, B virus antigen） in macaque monkey sera. The testing results were compared and analysed. Results Consistency of testing results of EIA （HSV-1 antigen）with DIA （ B virus antigen）, IFA （B virus antigen） and ELISA （B virus antigen）were 100%, 95.6% and 97.4%, respectively. The negative sera which were confirmed by EIA （HSV-lantigen） were still negative by IFA（ B virus antigen）. Among 779 negative sera confirmed by EIA （HSV-1 antigen）, 2.6 % （20） was positive by ELISA （B virus antigen）. Conclusion There is much consistency of testing results of EIA（ HSV-1 antigen）with DIA （B virus antigen）, IFA（ B virus antigen）and ELISA （B virus antigen）. Meanwhile, among B virus free animals which were confirmed using HSV-1antigen, very few animals would be positive using B virus antigen.
出处
《中国比较医学杂志》
CAS
2005年第4期237-239,共3页
Chinese Journal of Comparative Medicine
关键词
猴疱疹病毒
血清学试验
Herpesvirus simiae
Serologic tests
