摘要
中试制备小鼠神经生长因子 ,将小鼠颌下腺经研磨、匀浆、离心、除脂等步骤粗提后 ,经CM 32柱层析、Mr10 0 0 0膜超滤、CM sepharoseFastFlow柱等分离纯化后 ,平均每 10 0 0只成年雄性小鼠颌下腺可获得2 5S神经生长因子 (NGF) 110mg～ 130mg ,Mr 为 130 0 0± 2 0 0 0 ,等电点pH 8 6～ 9 3,结果表明 ,此法生产的NGF纯度及比活均有所提高 ,并且生产流程缩短。
To medium scale mice nerve growth factor,mice submaxillary glandwas ground, extracted,centrifugated and defatted successively,then,the mixture was purified by CM cellulose, ultrafiltration( M r 10 000), CM sepharose Thus 2 5S Nerve Growth Factor(NGF)was obtained The yield of this product was 110mg～130mg from 1000 male mice The M r was 13000±2000 and the isoelectric point was 8 6～9 3 The result showed that puring and specific activity of NGF is higher with this method,and the production process is shorter
出处
《药物生物技术》
CAS
CSCD
2000年第1期50-53,共4页
Pharmaceutical Biotechnology
