摘要
应用免疫细胞化学技术与图像分析系统研究三七皂苷的免疫药理学机制。正常组大鼠;实验组大鼠(左颈总动脉结扎并于术前4小时肌注三七皂苷,7mg/kg^(-1))与对照组大鼠(左颈总动脉结扎用等量生理盐水取代三七皂苷)各分4小时组、12小时组、24小时组。各组鼠大脑皮质、海马与尾壳核内神经元性一氧化氧合酶(nNOS)的含量用LSAB法与计算机数字图像分析系统检测。结果显示对照组大鼠海马、尾壳核与大脑皮质内nNOS含量显著下降,三七皂苷可恢复缺血脑组织的nNOS表达,并对缺血脑组织发挥保护作用。同时,本实验还提示免疫细胞化学技术与图像分析系统用于免疫药理学研究是可行的。
To understand the immunopharmacological mechanism of notoginsenoside, immunocy-tochemistry(LSAB)and image analysis system was employed in this experiment. Neuronal nitric oxide syn-thase (nNOS)was labelled and detected pualitariveoy and qualitatively in cerebral cortex, hippocampus and caudate putanen nuclei in normal rat brains . ischemic rat brains (experimental group )in postischemic peri-dos of 4h, 12h. 24h, which the notoginsenosied was administered prior to the occlusion of left commoncar-otid artery (LCA). Specimens for LSAB and image analysis were also collected from rats in control group at 4h, 12h and 24h, after the occlusion of LCA without injection of notoginsenoside. The content of nNOS in cerbral cortex, hippocampus, and caudate putamen nuclei of control rat brains decresed remarkably when compared with normal rats. Intensity of positive reaction to antiNOS-Iin ischemic rats was simil ar to that of normal rat brains. The results indicated that notoginsenoside could promote the cexpression of nNOS at the early stage of ceredral ischemia and suggested that notoginseoside might play a protective role fro nervous tissue in ischemic rains. Also, it was proved to be possible that immynocytochemistry and image analysis system could be a powerful approaches in the research of immunopharmacology as well.
出处
《脑与神经疾病杂志》
2000年第4期196-200,共5页
Journal of Brain and Nervous Diseases
关键词
脑缺血
一氧化氮合酶
三七皂苷
免疫细胞化学
Cerebral ischemia Neuronal nitric oxide synthase \Notoginsenoside Immunocy-tochemistry Image analysis system
