摘要
对从内蒙古奶豆腐及新生儿肠道中分离的5株植物乳杆菌的抗氧化活性进行分析。将保存的5株植物乳杆菌用MRS液体培养基活化培养后,对其耐酸特性、耐胆盐特性、疏水性和抗氧化能力进行分析。结果表明,5株植物乳杆菌均具有一定的耐酸和耐胆盐特性,其中植物乳杆菌MGSHC4302能够耐受pH=2.0的酸性环境,植物乳杆菌CRFA4608、MGSHC4301和MGSHC4302能够耐受1.0%的胆盐;5株植物乳杆对二甲苯和三氯甲烷的疏水性明显好于LGG,表明这些菌株具有较好的黏附性。实验结果表明,植物乳杆菌CRFA4608和MGSHC4302对羟自由基的清除能力高于对照菌株鼠李糖乳杆菌LGG,除植物乳杆菌MGSHC4303外其他4株菌对DPPH的清除能力均高于鼠李糖乳杆菌LGG或与之相近,而植物乳杆菌MGSHC4302、MGSHC4303具有较高的超氧阴离子清除能力。综上所述,植物乳杆菌MGSHC4302可以作为今后开发具有益生性产品的潜在菌株。
The in vitro probiotic potential of five Laaotacillus plantarum strains isolated fi＇om Inner Mongolia milk tofu and neonatal faeces was analysed, including acid tolerance, bile salt tolerance, cell surface hydrophobicity and antioxidative acitivity. The result shows that L. plantarum MGSHC4302 could survive 3h incubation at pH 2.0. L. plantarum strains CRFA4608, MGSHC4301 and MGSHC4302 were able to resist 1.0% bile salt. The hydrophobic character for dimethylbenzene and trichloromethane for L.plantarum strains were higher than LGG., it shows that these strains have good adhesity. L. plantarum CRFA4608 and MGSHC4302 showed higher hydroxyl free radical scavenging activity than the others. The L. plantarum strains showed higher DPPH radical scavenging activity than the control strain LGG except L. plantarum MGSHC4303. Strains MGSHC4302 and MGSHC4303 expressed excellent superoxide anion scavenging activity. In conclusion, L. plantarum MGSHC4302 showed good potential as probiotic for exploitation in the development of functional food.
出处
《中国乳品工业》
CAS
北大核心
2013年第4期10-14,共5页
China Dairy Industry
基金
现代农业产业技术体系建设专项资金资助(CARS-37)
吉林省科技厅科技成果转化项目(20115026)
关键词
植物乳杆菌
抗氧化性
耐酸
耐胆盐
Lactobacillus plantarum
inoxidizability
acid resistance
bile salts resistance
