摘要
目的:通过检测结肠腺癌组织中iNOS、eNOS和CD34的表达,探讨NOS与结肠腺癌血管形成的关系。方法:利用免疫组织化学SP法检测iNOS、eNOS和CD34在结肠癌不同病理分期中的表达,并观察其与肿瘤微血管密度的关系。结果:iNOS阳性率56.7％;eNOS阳性率53.3％;在结肠癌不同病理分期中,两种NOS表达具有差异性(P<0.05),同时微血管密度亦有差异(P<O.05),尤其是Dukes A期与Dukes C期相比较有显著差异性(P<0.01);eNOS阳性组中,NVD为40.9±6.21,阴性组中为29.8±8.27,二者相比有明显差异(P<0.05)。结论:结肠腺癌组织中iNOS、eNOS的表达与分期密切相关,对肿瘤的侵袭、生长起促进作用,内皮型NOS可能是促进肿瘤血管形成的主要参与者。
Objective: To investigate the expression of nitric oxide synthase(NOS) in clolon adenocarcinoma and correlation with tumor angiogenesis.Methods: The iNOS, eNOS and CD34 of 30 cases of colon adenocarcinoma were detected by immunohisto-chemical SP method,and the correlation between the expression rates and microvessel density( MVD) was observed. Results: The ex-pression rates of iNOS and eNOS were 56.7% and 53.3% respectively .There were significant difference between Dukes A、Dukes B and Dukes C cases. The expression of eNOS was significant associated with tumor angiogenesis.Conclusion: The expression of nitric oxide synthase(NOS) in colon adenocarcinoma was related to stage and promote tumor growth invasion. The expression of eNOS may contribute to the tumor angiogenesis.
出处
《临床肿瘤学杂志》
CAS
2000年第1期18-20,共3页
Chinese Clinical Oncology
关键词
结肠腺癌
一氧化氮
一氧化氮合酶
肿瘤血管形成
Colon adenocarcinoma Nitric oxide Nitric oxide syntheses Tumor angiogenesis
