摘要
建立基于非水胶束电动毛细管色谱在线富集技术测定蔬果中克百威和甲萘威残留的新方法。对影响分离和富集效果的缓冲液参数、分离电压和进样时间进行优化。运行缓冲溶液为含100mmol/L十二烷基硫酸钠、30mmol/L乙酸钠的甲酰胺-乙腈(85:15,V/V)溶液,表观pH值为9.4;分离电压20kV;进样时间150s。在此优化条件下,克百威和甲萘威的富集倍数分别为678和758倍;检出限分别为31μg/L和7.9μg/L;6次重复测定的峰面积相对标准偏差为2.47%、1.76%;平均加标回收率为80.4%～96.2%和92.5%～104.1%。以上结果表明该方法具有良好的灵敏度和重现性,可用于果蔬中该两种农药残留的检测。
A method of nonaqueous micellar electrokinetic capillary chromatography with online enrichment was developed to determine carbofuran and carbaryl residues in fruits and vegetables. The effects of buffer composition, apparent pH, applied voltage, and injection time were systematically discussed. The optimized buffer was composed of 100 mmol/L sodium dodecyl sulfate, 30 mmol/L sodium acetate, and formamide-acetonitrile （85：15, V/V）, and its apparent pH was adjusted to 9.4. The applied voltage was 20 kV and the sample injection time was 150 s. Under these optimal conditions, peak enrichment factor was 678 and 758; detection limit was 31 μg/L and 7.9 μg/L; RSD was 2.47% and 1.76%; average recovery rates of spiked samples were 80.4%-96.2% and 92.5%-104.1% for carbofuran and carbaryl residues, respectively. Therefore, this method is of high sensitivity, good repeatability and can be used in the determination of both pesticide residues in fruits and vegetables.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第8期176-181,共6页
Food Science
基金
教育部高等学校博士学科点专项(20093227110010)
江苏大学科研基金项目(08JDG001)
江苏高校优势学科建设工程资助项目
关键词
非水胶束电动毛细管色谱
克百威
甲萘威
甲酰胺
富集
nonaqueous micellar electrokinetic capillary chromatography
carbofuran
carbaryl residue
formamide
enrichment
