摘要
紫杉烯合成酶(Taxadienesynthase)被认为在紫杉醇合成代谢途径中起着限速酶的作用。为进一步研究紫杉烯合成酶的作用机理和紫杉醇生物合成代谢调控机制,采用RTPCR技术从东北红豆杉(Taxuscuspidata)愈伤组织中获得了紫杉烯合成酶基因片段,将该片段克隆在载体pGEMTEasyVector上,并转化到大肠杆菌JM109中,经EcoRI酶切检测,Southernblotting及部分cDNA序列分析证实该片段确为紫杉烯合成酶基因,与国外报道的从太平洋红豆杉(Taxus.brevifolia)幼茎中得到的紫杉烯合成酶基因序列具有很高的同源性。
Taxadiene synthase plays an important role in taxol biosynthesis.RT PCR was used for cloning taxadiene synthase cDNA fragment from the cells of T.cuspidata. The cDNA was cloned into vector pGEM and transformed to E.coli J M109.The cloned cDNA named pCBMZ was further confirmed by Southern blotting assay and was sequenced.The result showed that taxadiene synthase cDNA of Taxus cuspidata was highly homologous with that of Taxus brevifolia.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第2期158-160,共3页
Chinese Journal of Biotechnology
基金
国家教委"跨世纪优秀人才"基金
中国博士后科学基金
天津市 2 1世纪青年科学基金资助
