摘要
应用FLP重组酶介导的染色体定点整合技术,将带有不同拷贝数的乙肝病毒融合表面抗原SA28基因表达单元的质粒整合在酵母不同的染色体位点,并测定了SA28基因的表达情况,初步研究了基因拷贝数与染色体位置对酵母表达外源基因的影响。结果表明SA28基因在HIS3位点整合时的表达水平随基因拷贝数的增加而提高,遵循基因剂量效应;在某些染色体位点整合时,插入方向对其表达有不同程度的影响,呈现出明显的染色体极性效应。
The effect of gene copy number and chromosomal position on heterologous gene expression in Saccharomyces cerevisae has been tentatively investigated using a modified hepatitis B virus surface antigen SA 28 gene.The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated targeted integration.The expression of SA 28 gene in the resultant transformants was then measured.The result indicated that the expression of SA 28 gene was positively related to the gene dosage at HIS3 locus and had chromosomal polarity at several chromosomal loci.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第2期124-128,共5页
Chinese Journal of Biotechnology
基金
国家科委八六三高科技项目资助课题!( 86 3 10 2 0 7 0 1 0 2 1)
关键词
酵母
基因拷贝数
染色体位置效应
表达
Yeast
gene copy number
chromosomal position effect
hepatitis B virus surface antigen
FLP recombinase
