摘要
采用酵母杂合启动子PADH2－CUP1或PADH2－GAPDH及终止子TADH1，构建了一系列酵母表达载体．在这些表达载体中插入乙肝病毒表面抗原S－preS1融合基因SA－28后，将合乙肝病毒表面抗原的表达单元克隆至高稳定质粒PHC11的BamHI位点．然后将表达质粒分别转化酿酒酵母Y16，Y19．对SA－28基因表达的研究表明，在酵母菌胞内实现了SA－28基因的高表达，且表达受葡萄糖浓度调控．
Series of expression vectors were constructed, using hybrid promoters, PADH2-CUP1 or PADH2-GAPDH, and terminator TADH1. A modified antigen gene SA-28, coding for hepatitis B surface antigen (HBsAg) and preS1 epitope had been placed at down stream of these two hybrid promoters. After that, the expression cassettes were cloned into the BamH I site of a highly stable plasmid PHC11 to construct expression plasmids. Then they were transformed into two yeast strains, Y16 and Y19. A high level expression of S-preS1 was observed intracellularly, and the expression was regulated by the glucose concentration of the medium.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期439-444,共6页
Journal of Fudan University：Natural Science
基金
国家科委八六三高科技项目
