摘要
利用反转录 聚合酶链 (RT PCR)技术 ,扩增出禽呼肠孤病毒 (ARV )S113 3、173 3长为 5 40bp的S1基因片段。将扩增到的这 2个毒株的S1基因通过粘端连接分别克隆到pBluescriptⅡKS +质粒中 ,用PCR和限制性内切酶分析鉴定 ,表明获得 2种重组质粒ARVS113 3S1 pBluescript、ARV 173 3S1 pBluescript。
Using a reverse transcription polymerase chain reaction (RT PCR) the 540 bp long S1 gene of two strains (S1133、1733) of avian Reovirus (ARV) were amplified successfully. The S1 gene were cloned into plasmid pBluescript Ⅱ KS+ by ligation of cohesive end. And then the two recombinant plasmids of ARV S1133 S1 pBluescript and ARV 1733 S1 pBluescript were indentefied by PCR and restriction analysis.
出处
《中国兽医科技》
CAS
CSCD
2000年第8期3-5,共3页
Chinese Journal of Veterinary Science and Technology
基金
广西科技攻关项目
