摘要
从病鸡肝分离到一株病毒，经电镜检查、理化特性分析、核酸电泳和中和试验等证明它为禽呼肠孤病毒（ＡＲＶ）。该病毒只在鸡胚肝细胞（ＣＥＬｉ）上产生细胞病变（ＣＰＥ），在鸡胚成纤维细胞（ＣＥＦ）和Ｖ＿（ｅｒｏ）细胞上不增殖，它对热无敏感，Ｍｇ￣（２＋）不能增强其对热的稳定性，其基因组中的４个小节段的迁移率与ＡＲＶＦＤＯ和Ｓ＿（１１３３）株明显不同，表明它是不同于ＦＤＯ和Ｓ＿（１１３３）的ＡＲＶ变异株。
virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg￣(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.
出处
《中国病毒学》
CSCD
1996年第4期352-359,共8页
Virologica Sinica
