摘要
通过能量 30keV、剂量 7× 1 0 1 6 离子 /cm2 的Ar+注入 ,将两个大豆品种的全DNA(4 0 0 μg/ml)分别导入两个小麦栽培品种皖 92 1 0和扬麦 5号。经过离子注入和大豆DNA处理的种子发育成为植株后 ,当代未表现出明显不同于受体的变异性状。经转化处理的植株成熟后 ,按株分别收获脱粒 ,并对籽粒蛋白质含量等性状进行分析 ,发现有两个单株的蛋白质含量分别达到 2 0 46 %和 2 5 35 % ,明显高于受体。说明通过离子束介导外源DNA转化技术 ,可以绕过复杂的组培过程而由成熟种子直接发育为植株 ,也可获得带有目的性状的转化后代 。
The exogenous total DNAS of soybean were introduced into wheat breeds, Wan9210 and Yanmai 5, by Ar + implantation. The results showed that the dose of ions implanted was one of the important factors affected the germination and survival rate of wheat. The suitable dose was 7×10 16 ions/cm 2. The plants treated with Ar + and soybean DNA didn't show any apparent variation in the first generation. After harvest, the protein content of transformated progeny was analyzed. The protein content of two signle plants reached 20 46% and 25 35% respectively, which were much higher than that of control. It is concluded that low energy of ion beam mediated DNA delivery into mature seeds was a simple and effective method for the genetic material exchange of species with distant genetic relationship.
出处
《核农学报》
CAS
CSCD
2000年第4期206-211,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金!重大项目 (119890 30 0 )资助
国家重点科技攻关项目! (96 5 38 0 2 0 1)资助课题
