摘要
以2个雄器大多围生、少数侧生的苎麻疫霉菌株与1个雄器侧生、偶有围生的恶疫霉菌株为材料，采用真菌核糖体基因转录间隔区（ITS）通用引物，PCR扩增3个供试菌株核糖体基因的ITS1和ITS2，并对PCR产物进行了克隆和序列分析。结果是苎麻疫霉的ITS1和ITS2分别由206和453个碱基组成， 而恶疫霉则分别由218和415个碱基组成。2个供试苎麻疫霉菌株的ITS1和ITS2的碱基序列同源性均分别为100%。苎麻疫霉和恶疫霉ITS1同源性为74.9%，其中中间区域40bp-164bp之间在两种间变异丰富，同源性只有59.4%，而1bp-39bp和165bp-239bp两区域的同源性分别为92.3%和92.1%; ITS2在两种疫霉菌间的同源性为71.0%。结果表明苎麻疫霉和恶疫霉ITS的碱基序列有明显差异。上述结果提示，ITS区域碱基序列可区分苎麻疫霉和恶疫霉。
The configuration of antheridium is one of the most important biological characteristics for distinguishing Phytophthora boehmeriae from P. cactorum. In this paper the internal transcribed spacer regions, ITS1 and ITS2, of the ribosomal RNA gene of two isolates with partly paragynous antheridium of P. boehmeriae and one isolate of P. cactorum were amplified by polymerase chain reaction and sequenced. The total length of ITS1 and ITS2 in P.boehmeriae were 206bp and 453bp, respectively. In the tested isolate with paragynous antheridium of P. cactorum, the ITS1 was 218bp long, ITS2 415bp. Sequence analyses of ITS1 and ITS2 revealed no difference between two tested isolates of P. boehmeriae. However, there were so much difference between P. boehmeriae and P. cactorum. The homology of ITS1 between two species was about 74.9%, and the most difference was 40-164bp. Moreover, the ITS2 homology in two Phytophthora species was about 71.0%. This result indicated that the sequence difference within ITS region could provide an additional characteristic for distinguishing P. boehmeriae from P. cactorum.
出处
《菌物系统》
CSCD
北大核心
2000年第4期485-491,共7页
Mycosystema
基金
国家自然科学基金
教育部跨世纪人才基金资助
关键词
苎麻疫霉
恶疫霉
核糖体基因
碱基序列
分类
Phytophthora boehmeriae, Phytophthora cactorum, Antheridium attachment mode, Internal transcribed spacer (ITS), Sequence
