摘要
应用代谢流量平衡模型定量分析了杂交瘤细胞的代谢流量分布。结果表明 ,在连续培养的杂交瘤细胞中 ,当葡萄糖和谷氨酰胺的流加浓度分别为 13 8和 2 6mmol·L-1时 ,86 2 %的葡萄糖通过糖酵解生成乳酸 ,7 5 %的生成脂类 ,进入TCA循环的仅占 0 83% ;谷氨酰胺中的氮有 3%用于核酸的合成 ,5 4 5 %生成氨 ,另有 38 2 %生成非必需氨基酸 ,碳骨架 6 1 6 %生成非必需氨基酸 ,34 1%进入TCA循环。
Metabolic flux distribution of hybridoma cells in continuous culture with 13.8 mmol·L -1 glucose and 2.6 mmol·L -1 glutamine using metabolic flux balance model was analyzed quantitatively in this paper.Results showed that the 86.2% of glucose was consumed for lactate and 7.5% for lipids,0.83% of glucose was consumed in the TCA cycle.The 3% of nitrogen in glutamine was used for synthesis of nucleotide,54.5% of nitrogen was consumed for ammonia and 38.2% was used for synthesis of nonessential amino acids(NAA).The 61.6% of carbon skeleton of glutamine was consumed for NAA and 34.1% entered the TCA cycle.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第6期740-746,共7页
Chinese Journal of Biotechnology
基金
国家自然科学基金项目资助!( 2 960 60 11)
关键词
代谢流量分析
代谢网络
杂交瘤细胞
Metabolic flux analysis,metabolic network,hybridoma cells
