摘要
稻温病菌的分生孢子、芽管、附着胞的混合物作为抗原免疫BALB/c小鼠 ,取免疫小鼠的脾细胞与SP2 /0骨髓瘤细胞在 50 %PEG下融合成杂交瘤细胞 ,用间接ELISA筛选阳性孔 ,获 1 1株单克隆抗体。间接免疫荧光试验表明其中 4株单克隆抗体 2B4、4A1 、1D1 和 2H4分别与孢子、芽管或附着胞有特异性结合 ;Westernblotting分析发现 2B4、4A1 、1D1 单克隆抗体分别与孢子、芽管表面的提取物有不同的结合带 ;此四株单克隆抗体均干扰稻温病菌附着胞形成 ,并抑制稻温病菌在叶表的致病性。
Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies(McAbs)against Magnaporthe grisea were produced by fusing mouse myeloma cells(SP2/0)with spleen cells from BALB/c mice immunized with the mixture of conidia、germ tubes and appressoria of M.grisea using 50% PEG. The result of IFTC test showed that four of McAbs, named as 2B 4、4A 1、1D 1、and 2H 4 specificlly bound to the cell wall surface of the fungus; Western blotting revealed that 2B 4、4A 1、1D 1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrance and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第6期638-645,共8页
Acta Microbiologica Sinica
基金
国家自然科学基金支助项目!( 3 9670 0 1 1 )&&
