摘要
从浙江省豌豆和蚕豆病株上获得两个病毒分离物，编号为Ｐ－９３５和Ｂ－９３４．人工摩擦接种６科１９种植物，Ｐ－９３５能侵染４科１３种植物，Ｂ－９３４能侵染４科１４种植物，除菜豆外，它们在这些植物上的症状十分相似。Ｐ－９３５和Ｂ－９３４的钝化温度为５０－５５℃，稀释限点为１０￣（－３）－１０￣（－４），体外保毒期分别为５天和４天。两个分离物均能由桃蚜以非持久性方式传毒，用昆诺藜作繁殖寄主均提取到了大量病毒粒子，提纯病毒粒子球形，平均直径２５ｎｍ。病毒外壳蛋白均由两种亚基组成，分子量分别为４２．５ｋＤ和２１．２ｋＤ，感病叶片组织中观察到了无定形的泡状内含体，在琼脂双扩散试验中，Ｐ－９３５和Ｂ－９３４均能与蚕豆萎蔫病毒（ＢＢＷＶ）抗血清（血清型Ⅱ）形成清晰沉淀线。根据以上特性，Ｐ－９３５和Ｂ－９３４均被鉴定为蚕豆萎蔫病毒，且都属血清型Ⅱ．初步调查表明杭州郊区豌豆病毒病均由ＢＢＷＶ引起，蚕豆病毒病主要有ＢＢＷＶ和黄瓜花叶病毒（ＣＭＶ）。
Two virus, isolates,P-935 and B-934 ,were obtained from diseased Pisum sativum and Vicia faba,and 14 plant species in 6 families were mechanically inoculated by the isolates.Among the tested plants,P-935 could infect 13 species and B-934 infected 14 species. The two isolates pro-duced similar symptoms on these plants,except for symptoms on Phaseolus vulgaris.The two Iso-lates were easily transmitted by Myzus persicae in a nonpersistent manner.P-935 and B-934 were purified form Chenopodium quinoa by a method that yielded up to 147 mg/kg and 1 28 mg/kg tis-sue,respectively. Purified preparation of two isolates contained isometric particles ca. 25 nm in di-ameter,The capsid protein contained two polypeptides with molecular weight of 42.5 kD and 21.2 kD. Amorphous inclusions with membrane accumulations were found in the cytoplasm of infected Pisum sativum leaf tissue. In agarose gel diffusion tests,P-935 and B-934 strongly reacted with the antisera of BBWV serotype Ⅱ.On the bases of these characteristics,P-935 and B-934 were identi-fied as broad bean wilt virus(serotype Ⅱ).Field survey in Hangzhou surburb showed viral dis-eases were severe in Pisum sativum and Vicia faba.
基金
国家自然科学基金
关键词
豌豆
蚕豆
萎蔫病毒
生物学
血清学
鉴定
Pisum sativum
Vicia faba: broad bean wilt virus
biology
serology
identification
