摘要
为了从分子水平上检验野生棉遗传特性向陆地棉转育的结果以及育成种质之间的遗传差异，通过等电聚焦电泳和RAPD技术，对来自科遗181陆地棉品系与野生比克氏棉杂交后代的6个遗传稳定的不同种质系及其亲本的过氧化物酶同工酶图谱和DNA指纹图谱进行了分析。结果如下：（1） 6个种质系的基本酶谱特征均倾向于陆地棉亲本，但在其中2个种质系的过氧化物酶图谱中，观察到各具有1条等电点值（PI）为4.85的野生亲本的特征带；（2） DNA指纹图谱的分析表明，不同种质系之间在基因组水平上具有高度的异质性。并且在OPO10和OPO11 2个引物的扩增图谱中观察到4个育成种质系具有野生比克氏棉亲本的特征带。
To study the effectiveness of Gossypium interspecific hybrid breeding on molecular level, the six germplasm lines from interspecific hybrids between G. hirsutum and G. bickii were analysed by the techniques of RAPD and isozyme electrophoresis. The main results were summarized as follows: (1) The basic zymograms of peroxidase isozyme of the six germplasm lines were similar to their G. hirsutum parent, but two out of the germplasm lines showed one specific band of the wild parent of G. bickii (pI 4.85); (2) The RAPD fingerprint showed distinct difference of genomic DNA among the germplasm lines; (3) The four specific DNA fragments of G. bickii were observed in RAPD patters of four out of the six germplasm lines.
基金
中国科学院"九五"重大攻关项目!(批准号:KZ951-A1-101-03-06)
