摘要
白僵菌是桑天牛的生物防治菌种之一,探究白僵菌菌种退化的遗传机理有助于利用生物工程技术控制菌种退化,提高其生防效率。利用RAPD技术分析第1、6、12、14、16、18和20代白僵菌菌株在基因水平的变异情况,结果表明各代菌株间既有共有的条带,同时又有谱带的增加或减少,与第1、6代菌株相比,第12代菌株的基因组DNA发生了明显的变化,第14代时继续变化,之后的第16、18和20代菌株又趋于稳定。供试的7代菌株间的遗传距离变化范围在0.05～0.60之间,存在较明显的遗传差异,且随传代次数增加,菌株的菌丝生长量增大,产孢能力降低和对桑天牛幼虫致病能力降低等检测结果具有一定的一致性。研究结果提示,可利用RAPD技术进行桑天牛幼虫致病性白僵菌菌株退化的早期遗传检测。
Beauveria bassiana is an important biocontrol strain against Apriona germari.The study about genetic mechanism of Beauveria bassiana degeneration would facilitate control of strain degeneration using biological engineering technology and enhance biocontrol efficiency.In this study,variations of the 1st,6th,12th,14th,16th,18th and 20th generations of B.bassiana strain were analyzed at the gene level using RAPD technology.The results showed that each generation not only has the common bands,but also has additional bands or missing bands.Compared with the 1st and 6th generations,the genomic DNA of the 12th generation has changed obviously,and that of the 14th generation continued such change till the 16th,18th and 20th generations of which genomic DNA was stabilized.Genetic distances among the tested 7 generations varied within the range of 0.05～0.60,showing obvious genetic difference which is consistent with analytical results on increase of mycelium growth,reduction of sporulation yield,and decline of pathogenicity to A.germari larvae with the increase of generation number.These results showed that RAPD technology can be used to conduct early genetic determination on degeneration of B.bassiana for biocontrol of A.germari larvae.
出处
《蚕业科学》
CAS
CSCD
北大核心
2013年第2期226-230,共5页
ACTA SERICOLOGICA SINICA
基金
河北省自然科学基金项目(No.C2011204041)
河北省强势特色学科资助项目(No.2011003)
关键词
白僵菌
传代
RAPD分析
菌丝生长量
产孢力
致病力
Beauveria bassiana
Degeneration
RAPD analysis
Mycelial growth
Sporulation yield
Pathogenicity
