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Purification and characterization of schizophyllan from Schizophyllum commune

Purification and characterization of schizophyllan from Schizophyllum commune
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摘要 The schizophyllan from Schizophyllum commune was purified and characterized. The crude schizo-phyllan was obtained from Schizophyllum commune fermentation broth by decoloration with activated carbon,followed by deproteinization with Sevag method and ethanol precipitation. The pure schizophyllan was obtained by gel filtration chromatography with Sephacryl S-500,and its molecular characteristics were examined. The result showed that the molecular weight was 3.8×104 ,and the schizophyllan consisted of glucose with β-(1→6)-glucosidic linkages.
出处 《Engineering Sciences》 EI 2013年第2期88-92,共5页 中国工程科学(英文版)
基金 National Nature Science Foundation of China (No.31171662) "973"National Key Basic Research and Development Program(No.2012CB518202) Project of Qinghai Development of Science and Technology(No.2011-N-150) Key Project of GLD of PLA
关键词 SCHIZOPHYLLAN Schizophyllum commune PURIFICATION CHARACTERIZATION 纯化 凝胶过滤色谱法 表征 Sevag法 乙醇沉淀 分子特性 裂褶多糖 活性炭
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