摘要
为了明确浙南地区小麦育种亲本资源的遗传多样性,利用分布于小麦21条染色体上的88对SSR引物对48份小麦品种资源基因组DNA进行扩增,结果显示:其中有27对引物在48份材料之间具有稳定的多态性,共检测出159个等位位点,每对引物等位位点数为2～9个,平均为5.89个。位点多态信息含量(PIC)变幅为0.117～0.850,平均为0.652。48份小麦品种(系)间遗传相似系数(GS)变幅为0.68～1.00,品种资源间的遗传多样性较低。采用非加权类平均法进行聚类分析,在相似系数0.75处,可将48份小麦品种(系)分为6大类群,在一定程度上反映了品种资源之间的亲缘关系。
To elucidate the genetic diversity of wheat breeding parents in southern Zhejiang province, 88 SSR mark- ers located on 21 chromosomes of wheat were amplified to study the genetic diversity among 48 wheat euhivars. Sta- ble polymorphism could be amplified by 27 out of 88 SSR markers among 48 wheat euhivars. A total of 159 alleles were identified in these varieties. Each of primer pairs could detect 2 to 9 alleles, with an average of 5.89. The PIC value was in the range from 0. 117 to 0. 850, with the average of O. 652. The genetic similarity coefficients of the 48 wheat eultivars ranged from O. 68 to 1.00, which suggested that the diversity of these 48 wheat cultivars was lower. Fur- thermore, all the cultivars were classified into 6 groups at the level of genetic similarity coefficient of 0. 75 by un-weighted pair-group method with arithmetic mean. The results of grouping can reveal the genetic relationship of cultivars.
出处
《浙江农业学报》
CSCD
北大核心
2013年第2期219-224,共6页
Acta Agriculturae Zhejiangensis
基金
上海市农业生物基因中心基金项目(沪农基201001)
温州市科技局项目(N20100059)
