摘要
采用１６Ｓ ｒＤＮＡ－ＲＦＬＰ技术，对自新疆土壤中捕捉的３４株快生大豆根瘤菌及相关己知种的模式菌株进行了比较分析。从酶切图谱类型和聚类结果表明，所有新分离的菌株与Ｓ．ｘｉｎｊｉａｎｇｅｎｓｉｓ的图谱类型基本一致，而与Ｓ ．ｆｒｅｄｉｉ的图谱类型有明显差异，与Ｓ． ｍｅｌｉｌｏｔｉ； Ｓ． ｓａｈｅｌｉ， Ｓ． ｍｅｄｉｃａｅ， Ｓ． ｔｅｒａｎｇａ也不相同。 ３４株新分离的菌株全部与Ｓ．ｘｉｎｇｊｉａｎｇｅｎｓｉｓ菌株在９０％的相似性水平上聚在一起。再一次肯定了Ｓ． ｆｒｅｄｉｉ与Ｓ． ｘｉｎｊｉａｎｇｅｎｓｉｓ快生大豆根瘤菌属于两个不同的类群，为两个独立的种。我们还将用更多的分析方法进一步证实他们的分类地位。
Thirty-four fast-growing soybean rhizobial strains isolated from Xinjiang Regions were analyzed by 16S rDNA-RFLP in comparison with several type and reference strains of related species. The results indicated that all 34 isolates shared the same patterns and above the similarity lever of 90% clustered with S. xinjiangensis strains, but were different with S. .fredii, S. meliloti, S. saheli, S medicae and S. teranga. It showed that S. xinjiangensis is different from S.,fredii and should be assigned to a separated species according to the 16S rDNA-RFLP analysis. In order to make sure, the more analysis for the taxonomic position is under way.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第4期237-241,共5页
Microbiology China
