摘要
应用地高辛标记的对虾白斑杆状病毒 (whitespotsyndromebaculovirus,WSSV)核酸探针 ,与人工感染后不同时间采集的对虾组织样品进行原位杂交 ,以动态研究病毒从侵染至对虾发病死亡的过程。将典型感染WSSV的病虾组织投喂健康对虾 ,结果显示 :WSSV首先通过侵染消化道上皮进入虾体内增殖 ,此后随着细胞裂解、病毒粒子释放 ,游离的病毒粒子伴随血淋巴循环进而侵染其它靶组织 ,直至对虾发病死亡。
The tissue sections of artificially infected shrimp (Penaeus monodon) sampled at different times were hybridized in situ with digoxigenin (DIG)-labeled WSSV DNA probe in order to dynamically study the viral infection process in shrimp body. Feeding the healthy Penaeus monodon with diseased shrimp tissues which was confirmed to be typically infected with WSSV. The results showed that WSSV firstly infected and replicated in the epithelial cells of alimentary tract, the viral particles then released with the host cell lysis. The dissociative viral particles proceeded to infect other target tissues following hemolymph circulating in the shrimp body till the shrimp got diseased or died.
出处
《病毒学报》
CAS
CSCD
北大核心
2000年第3期242-246,共5页
Chinese Journal of Virology
基金
中国科学院重大B项目!(K2 95 1-13 1-111)
关键词
原位杂交
对虾白斑杆状病毒
斑节对虾
杂过程
hybridization in situ
Penaeus monodon
white spot syndrome baculovirus (WSSV)
infection process
