摘要
从患暴发性传染病的中国对虾组织中分离了一种病毒，经差速离心、密度梯度离心及Ｓｈｅｐｈａｒｏｓｅ２Ｂ柱层析纯化了一种形状为杆状的病毒。提纯病毒核酸电泳为一条带，分子量为２０ｋｂ。将提纯病毒免疫新西兰兔获得较高效价的抗血清并初步建立了酶联免疫吸附测定（ＥＬＩＳＡ）检测方法。可在六小时内完成对样品的检测，检测灵敏度达６０纳克（ｎｇ）。
A baculovirus(288×62.5nm) was isolated from hepatopancreas of infected Penaeus chinensis during an epizootic in Shanghai suburbs.The virus was purified by gradient centrifugationand Sepharose 2B chromatography.The viral nucleic acid is DNA of m.w.ca.20kb by agarose gel electrophoresis.An antiserum was prepared and indirect ELISA developed for the virus detection in the penaeid shrimp with gratifying results.
出处
《水产学报》
CAS
CSCD
北大核心
1995年第4期315-321,共7页
Journal of Fisheries of China
