摘要
目的探讨神经节苷脂（monosialotetrahexosy一1 ganglioside，GMl）对脊髓损伤诱导的神经元自噬性死亡的影响。方法90只sD大鼠按随机数字表法分为假手术组、等渗盐水组和GMl组，每组30只。等渗盐水组和GMl组采用Allen法建立T_。脊髓损伤动物模型，荧光显微镜下观察内源性细胞自噬相关蛋白（LC3）的表达；Western印迹法检测LC3—1I／LC3-I比值、细胞蛋白（Beclin-1）表达。结果与假手术组比较，等渗盐水组LC3表达水平明显上调（P〈0．05）；与等渗盐水组比较，GMl组LC3表达水平明显下调，自噬体形成明显减少（P〈0．05）。与假手术组比较，等渗盐水组LC3一I／、Beclin一1表达水平以及LC3—Ⅱ／LC3-I比值均明显上调（P〈0．05）；与等渗盐水组比较，GMl组LC3—11、Beclin-1表达水平以及LC3-I／／LC3-I比值均明显下调（P〈0．05）。结论GMl通过抑制脊髓损伤后自噬性细胞死亡，起到保护神经元作用。
Objective To investigate effect of monosialotetrahexosy-1 ganglioside （ GM1 ） on the autophagic neuronal death induced by spinal cord injury. Methods Ninety SD rats were randomly di- vided into sham group, isotonic saline group and GM1 group, with 30 rats per group. Spinal cord injury at Tt0 segment was induced by Allen method in the isotonic saline group and GM1 group. Expression of en- dogenous LC3 was detected by florescence microscope. Ratio of LC3-[[ and LC3- [ , and expression of Beclin-1 were detected by Western blot. Results LC3 was significantly up-regulated in the isotonic sa- line group, as compared to the sham group （ P 〈 0.05 ）. Also, an up-regulation of LC3 and a decline of autophagic body formation were observed in GM1 group, as compared to isotonic saline group （P 〈 0.05 ）. Expressions of LC3-11 and Beclin-1 and ratio of LC3-11 to LC3- I were significantly increased in the isotonic saline group, as compared to the sham group （ P 〈 0.05 ）. Expressions of LC3-1I and Beclin- 1 and ratio of LC3-I1 to LC3- I significantly were decreased in the GM1 group, when compared to the i- sotonic saline group （ P 〈 0.05 ）. Conclusion GM1 inhibits autophagic neuronal death after spinal cord injury and hence exerts protective effect on the neurons.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2013年第4期372-375,共4页
Chinese Journal of Trauma
