摘要
采用吖啶橙(AO)与溴化乙啶(EB)荧光双染色以及Hoechst 33258荧光染色法对HMC毒素诱导玉米B37-C、B37-N根冠细胞凋亡率进行检测。结果表明,采用AO/EB双染色后,当HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C根冠细胞凋亡率分别为24.8%、58.2%、70.2%,B37-N仅为11.0%、25.7%和36.6%;当浓度为150μg/mL,处理时间4 h时B37-C根冠细胞最大凋亡率为62.3%,B37-N为25.8%。经Hoechst 33258染色后,HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C的根冠细胞凋亡率分别为32.5%、58.7%、74.5%,B37-N仅为7.5%、22.3%和30.7%;HMC毒素浓度为150μg/mL,处理4 h时B37-C的细胞凋亡率为62.3%,B37-N为19.3%。
AO and EB fluorescent double staining and Hoechst 33258 fluorescent staining methods were used to detach the apoptosis of root cap cells. Detection of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from a pair of homokaryon B37-C and B37-N inbred line of maize. In contrast, the ratios of apoptosis of B37-C were 24.8%, 58.2%, 70.2%, and B37-N were 11.0%, 25.7%, 36.6% by AO and EB staining, after HMC-toxin with 50, 100, 150 μg/mL treated for 7h. The max ratios of apoptosis of B37-C and B37-N stained by AO and EB were 62.3% and 25.8%, respectively, after HMC toxin with 150 μg/mL treated for 4 h. And the ratios of apoptosis of B37-C were 32.5%, 58.7%, 74.5% and B37-N were 7.5%, 22.3%, 30.7% by Hoechst 33258 staining, after HMC-toxin with 50, 100, 150 μg/mL treated for 7 h. The max ratios of apoptosis of B37-C and B37-N stained by Hoechst 33258 were 62.3% and 19.3%, respectively, after HMC toxin with 150 μg/mL treated for 4 h.
出处
《玉米科学》
CAS
CSCD
北大核心
2013年第2期98-101,共4页
Journal of Maize Sciences
基金
河北省自然科学基金(C2011301010)
河北省高层次人才"三三三人才工程"项目(A2010003028)
河北省科技计划项目(13396401D)
科技部国际合作项目(2006DFB02480)
农业部948重点项目[2011-G1(3)-07]
关键词
玉米
小斑病菌C小种
毒素
细胞凋亡
荧光染色
Maize
Helminthosporium maydis race C（HMC）
Toxin
Apoptosis
Fluorescent staining
