摘要
利用低温人工气候箱,设置0℃、1℃、2℃、3℃共4种胁迫温度,2、4、6、8 d共4种胁迫时间,采用4×4交互式设计的方法,对来自国内外36份玉米自交系进行芽期耐寒性强度的研究,建立玉米自交系芽期耐寒性鉴定方法,为耐寒遗传育种研究提供优异基因资源。结果表明,随着低温胁迫的增强,各玉米自交系的平均存活率都随之降低,以2℃/6 d低温胁迫下的36份玉米自交系存活率的标准差和变异系数表现为最大,该低温胁迫强度对于玉米自交系芽期耐寒性鉴定是比较合适的胁迫条件。在2℃/6 d低温胁迫下,筛选出强耐寒自交系7份,中度耐寒自交系11份,敏感自交系17份,高度敏感自交系1份。来自俄罗斯的玉米自交系芽期耐寒性显著强于来自其他地区的自交系,从中挖掘极强耐寒种质资源的潜力较大。
In order to propose identification method of chilling tolerance at the budding stage and provide the excellent genetic resources for breeding of chilling tolerance in maize, the chilling tolerance for 36 maize inbred lines from home and abroad at the budding stage were evaluated under the artificial climate chamber for low temperature where cultivars were exposed to 0℃, 1℃, 2℃ or 3℃. Exposures lasted for 2 days, 4 days, 6 days and 8 days. The results showed that the survival rate of maize inbred lines dramatically decreased with increasing stress intensity. The standard deviation and variation coefficient of the survival rate were maximum at 2℃ for 6 days, so this chilling stress was applied to the identification for chilling tolerant maize inbred line. Among 36 inbred lines, 7 lines with high chilling tolerance, 11 lines with medium chilling tolerance, 17 lines with chilling susceptible, one lines with high chilling susceptible. The chilling tolerance at the budding stage of maize from Russia was significantly stronger than those from other areas. It is of larger potential to select excellent resources with very strong chilling tolerance.
出处
《玉米科学》
CAS
CSCD
北大核心
2013年第2期88-92,共5页
Journal of Maize Sciences
基金
国际科技合作项目(2011DFR30840)
关键词
玉米
自交系
芽期耐寒性
鉴定方法
Maize
Inbred line
Chilling tolerance at the budding stage
Identification method
