摘要
目的:观察补肾活血中药对大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型初级视皮质(primary visual cortex,PVC)脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的干预作用,并对其作用机理进行初步探讨。方法:采用烙闭上巩膜静脉法,烙闭大鼠3支上巩膜静脉,制作大鼠慢性EIOP模型,随机分为3组:模型组,给药组,空白组。连续灌胃8wk,并于8wk末处死大鼠,观察其对EIOP大鼠眼压、PVC的BDNF表达及神经元细胞超微结构的影响。结果:本实验采用的烙闭上巩膜静脉的造模方法使大鼠眼压明显升高(P<0.01),与造模前比较差异有显著统计学意义(P<0.01);PVC病理切片半定量分析表明,模型组BDNF总面积82438±2597.39(S/μm2)、平均光密度1155.9±123.14、积分光密度12915±673.28,与空白组(总面积132370±7588.47S/μm2,平均光密度5365±379.65,积分光密度35102±2648.5)比较均有显著统计学意义(均为P<0.05),模型组BDNF总面积与给药组(108980±9126.77S/μm2)比较有统计学意义(P<0.05),给药组平均光密度(3220.4±413.67)与模型组比较有统计学意义(P<0.05),给药组积分光密度(23821±3431.68)与模型组比较,差异有统计学意义(P<0.05)。结论:补肾活血中药通过增强BDNF的表达、改善神经元细胞超微结构而促进EIOP大鼠初级视皮质损伤的修复。
AIM. To observe the effect of traditional Chinese medicine （TCM） of bushenhuoxue on primary visual cortex （PVC） brain- derived neurotrophic factor （BDNF） in rat model of chronic elevated intraocular pressure （ EIOP）, and explore the mechanism of it initially. METHODS： The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral veins, then 30 rats were divided into 3 groups randomly： control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure （ lOP）, expression of BDNF and ultrastructure of neuron cell in the PVC was observed. RESULTS. Unilaterally cauterizing episcleral veins increased lOP of the rat model obviously, there was significant difference compared with pre- operation （ P〈 0.01 ）. Semi- quantitative pathological analysis on PVC showed that BDNF of total area in the model group was （82438±2597.39） S/μm2, mean optical density was （ 1155.9±123.14）, integrated optical density was （ 12915 ± 673.28）, compared with the control group t total area was （132370± 7588.47） S/μm2, mean optical density was （5365±379.65）, integrated optical density was （35102 ± 2648. 5） }, there were statistical differences （all P〈 0. 05）, there was statistical difference in BDNF of total area between model group and treatment group t （108980±9126.77） S/μm2, P〈 0.05）, significant difference in mean optical density between the model group and treatment group （3220.4± 413.67, P〈O. 05）, statistical difference in integrated optical density between the model group and treatment group （23821 ±3431.68, P〈0.05）. CONCLUSION. TCM of bushenhuoxue can repair the PVC damage in the rat model of chronic EIOP by enhancing expression of BDNF, improving ultrastructure of neuron cell.
出处
《国际眼科杂志》
CAS
2013年第4期647-651,共5页
International Eye Science
基金
Key Projects of Sichuan Provincial Education Office, China (No.10ZA096)~~
关键词
青光眼
大鼠慢性高眼压模型
补肾活血法
初级
视皮质
尼氏体
神经元细胞超微结构
glaucoma
rat model of chronic elevated intraocular pressure
TCM of bushenhuoxue
primary visual cortex
nissl bodies
ultrastructure of neuron cell
