摘要
对产恩拉霉素的杀真菌链霉(Streptomyces fungicidious)摇瓶发酵培养基和发酵条件进行了优化,确定了最优恩拉霉素发酵培养基(%):葡萄糖2.0,可溶性淀粉4.0,玉米浆1.0,麸质粉3.0,冷榨黄豆粉1.5,NH_4Cl 0.5,MgSO_4 0.01,NaCl 1.5,异亮氨酸0.2,CaCO_3 2.0。最优摇瓶发酵条件:培养温度28℃,摇床转速225 r/min,初始pH值7.0,摇瓶装量为80 mL/750 mL,发酵时间8.5 d。优化后恩拉霉素的发酵效价与初始培养基相比提高了约6倍。
In order to improve enramycin production, both fermentation medium and fermentation conditions in flask were optimized. The optimum culture determined was as follows ： glucose 2.0, soluble starch 4.0, corn steeping liquor 1.0, corn gluten meal 3.0, cold pressed soybean flour 1.5, ammonium chloride 0.5, magnesium sulfate 0.01, isoleucine 0.2, sodium chloride 1.5, calcium carbonate 2.0. The optimum fermentation conditions were ascertained ： culture temperature 28 ℃, rotation speed 225 r/min, pH 7.0 before sterilization, 80 mL medium in 750 mL flask, fermentation time 8.5 d The last fermentation titer was improved approximately 6 times than that of the original.
出处
《工业微生物》
CAS
CSCD
2013年第2期64-68,共5页
Industrial Microbiology
基金
国家自然科技基金(81072557
81273413)
国家"重大新药创制"科技重大专项资助项目(No.2012ZX09201101-008)
关键词
恩拉霉素
优化摇瓶发酵工艺
发酵培养基
发酵条件
enramycin
optimization of flask fermentation process
fermentation medium
fermentation conditions
