摘要
目的:建立蜜麸炒僵蚕的质量标准。方法:按照中国药典规定的相应方法进行显微鉴别、水分测定、灰分测定、浸出物测定和含量测定研究,以CIE-LAB色度值为指标,采用色彩色差计测量僵蚕炮制前后颜色的变化,薄层鉴别在已有方法基础之上适当改进,效果良好。结果:研究表明,僵蚕炮制前后显微鉴别特征变化不大。炮制后水分含量约为6.0%,总灰分含量均值为5.4%,酸不溶性灰分含量为0.12%,水溶性浸出物含量均值为21.4%,醇溶性浸出物含量为13.44%。薄层鉴别显示炮制品和生品甲醇提取物没有太大差别,但色彩差别较明显,蜜麸炒僵蚕的总色度值均值为47.269,而生品僵蚕的总色度值的均值为60.595。以L*,a*,b*值为指标,蜜麸炒僵蚕的L*值的数值范围为45.984±3.998,a*值的数值范围为8.315±2.149,b*的数值范围为8.949±3.322。蜜麸炒僵蚕中指标性成分草酸铵的含量初步定为不低于4.0%。结论:除色差鉴别外,本质量标准的各项要求及结果均在中国药典规定的生品僵蚕质量标准范围内,因此可作为蜜麸炒僵蚕质量标准研究的参考。
Objective： To establish the quality standard of honey bran fried Bombyx Batryticatus. Method： According to the provisions of the Chinese pharmacopoeia methods for microscopic identification, the moisture content, ash content, extract and content were determined. Adopted the chroma difference meter to measure chroma changes before and after processed by using the CIE-LAB chroma value as indicators. The TLC identification was improved based on reference methods and the effects were good. Result： The research results showed that the processed products and raw products had the same microscopic characteristics, and the moisture content was about 6.0% , the total ash content mean was 5.4% , the acid insoluble ash content was 0. 12% , water- soluble extract content mean was 21.4% , alcohol-soluble extract content was 13.44%. The thin layer identify showed that the methanol extracts of processed products and raw products were almost not different from each other. But the color difference was quite evident. The processed products＇ total chrominance mean value was 47. 269, and the raw products＇ total product color value of the mean was 60. 595. In L＊ , a＊ , b ＊ value as indicators, the honey bran fried products from the numerical value of the L＊ range of 45. 984 ± 3. 998, a＊ value is in the range of 8. 315 ±2. 149, b＊ value was 8. 949 ± 3. 322. The content of ammonium oxalate in honey bran fried Bombyx Batryticatus should not be less than 4.0%. Conclusion： This quality standards and regulations are both in therange of the product quality standard of Bombyx Batryticatus in China pharmacopoeia except of the color identification. So it can be used as a honey stir frying with bran quality standard of reference.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第8期120-125,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省中医药管理局项目(2009012)
关键词
僵蚕
炮制
蜜麸炒
质量标准
Bombyx Batryticatus
processed technology
honey-bran fried
quality standards
