摘要
目的探讨体外序贯酶消化法分离大鼠髓核细胞的可行性,为体外获取纯度较高的NPCs提供理论基础。方法采用序贯酶消化法分离培养大鼠NPCs,倒置相差显微镜下观察细胞的黏附、增殖及细胞形态的变化。结果序贯酶消化法分离出的NPCs经多次换液及传代后得到形态比较一致的典型的多角形细胞,细胞呈漩涡状、岛状、集落样生长。结论序贯酶消化法能分离出纯度较高的NPCs,是体外培养髓核细胞成熟有效的方法。
Objective To explore the feasibility of isolating and cultivating rat nucleus pulposus cells（NPCs）by the sequential enzyme method in vitro,providing a theoretical basis for obtaining high purity NPCs.Method Isolateing and cultivateing rat nucleus pulposus cells by the sequential enzyme method;observing cell adhesion,proliferation and cell morphology changes by inverted phase contrast microscope.Result Nucleus pulposus cells were isolated by the sequential enzyme method after repeated change medium and passage,and cells presented typical nucleus pulposus cells morphology,and were closely packed like whirlpool,island,colony.Conclusion The sequential enzyme method was able to isolate NPCs in higher purity,and it was an effective method culturing rat nucleus pulposus cells in vitro.
出处
《九江学院学报（自然科学版）》
CAS
2012年第3期70-73,共4页
Journal of Jiujiang University：Natural Science Edition
基金
国家自然科学基金计划项目(项目批准号81160226)成果之一
关键词
序贯酶消化法
髓核细胞
分离培养
sequential enzyme method
NPCs
isolate and culture
