摘要
目的:探讨解毒化瘀方对HL60/VCR白血病细胞耐药株多药耐药逆转机制。方法:通过制备含解毒化瘀药的兔血清培养HL60/VCR白血病细胞耐药株后,用免疫组化法检测细胞的多药耐药基因P-gp。结果:解毒化瘀方含药血清能下调HL60/VCR白血病细胞耐药株的P-gp表达。结论:解毒化瘀方逆转HL60/VCR白血病细胞耐药株多药耐药与其能下调P-gp的表达有关。
Objective： To study the reversion of MDR mechanism of Detoxification and Dissipation Blood Stasis Formula on HL60/VCR leukemic cell drug resistance strains.Method： HL60/VCR leukemic cell drug resistance strains were cultured with rabbit serum containing Detoxification and Dissipation Blood Stasis Formula, then the MDR gene P-gp was detected by immunohistochemistry method.Result： The serum containing Detoxification and Dissipation Blood Stasis Formula could down-regulate the expression of P-gp Of HL60/VCR leukemic cell drug resistance strains.Conclusion ： The reversion of MDR of Detoxification and Dissipation Blood Stasis Formula on HL60/VCR leukemic cell drug resistance strains is related to that it could down-regulate the expression of P-gp.
出处
《实用中医药杂志》
2013年第4期241-242,共2页
Journal of Practical Traditional Chinese Medicine
关键词
解毒化瘀化方
逆转
多药耐药
P-GP
Detoxification and Dissipation Blood Stasis Formula
reversion
MDR
P-gp
