摘要
目的:观察槲皮素对兔离体肠平滑肌肌张力的影响,并探讨其作用机制。方法:试验采用经典的离体小肠灌流技术,观察槲皮素(Que)对肠平滑肌自主收缩活动以及乙酰胆碱(Ach)、氯化钡(Bacl2)及组胺所致痉挛性收缩肠平滑肌的影响。应用L型钙通道开放剂Bay K8644、肌浆网ryanodine受体阻断剂钌红(RR)、IP3受体阻断剂肝素(HP)和一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME),研究槲皮素舒张肠平滑肌作用的机制。结果:槲皮素(20、40、60、80、100μmol/L)能剂量依赖性的抑制家兔离体小肠平滑肌自发性收缩,对乙酰胆碱,组胺和氯化钡所致的兔离体肠痉挛性收缩也具有剂量依赖性抑制作用,且与肠平滑肌自发性收缩比较差异有显著性(P<0.05或P<0.01)。槲皮素可明显抑制Bay K8644(0.5μmol/L)引起的肠平滑肌收缩,HP能减弱槲皮素舒张肠平滑肌作用(P<0.05),而RR对此无明显影响(P>0.05)。L-NAME(100μmol/L)亦能够部分阻断槲皮素舒张平滑肌作用(P<0.05)。结论:槲皮素明显抑制了家兔离体小肠平滑肌的收缩活动,其机制与增加小肠平滑肌NO浓度,抑制外钙内流和IP3受体途径介导的内钙释放有关,但对肌浆网ryanodine受体途径引起内钙释放无明显影响。
Objective： To observe the influences of quercetin（Que） on the contraction of small intestine smooth muscle of rabbits/n v/tro and explore the mechanism. Methods： With the isothermal peffusion of small intestine in vitro. The influences of quercetin on the spontaneous contraction of small intestine and contraction induced by Ach, histamine and Bacl2 were observed and the mechanism of quercetin was studied. Resulls： Quercetin reduced the tension of contraction of small intestine smooth muscle in rabbits in a dose---depended manner. Quercetin could completely block the contraction of Bay K8644. Heparin could also block the inhibition of quercetin on small intestine smooth muscle but ruthenium red（RR） had no effect on the relaxation of quercetin. Nitro-L-arginine methylester（L-NAME） inhibited the relaxition of quercetin. Conduon： Quercetin inhibits the contraction of small intestine smooth muscle of rabbits in vitro. The mechanism may be related to increase NO concentration in smalI intestine smooth muscle so that it inhibits exacellular Ca2 ＋ inflowing v/a cell membrane . And quercetin has effect on intracellular Ca2 ＋ releasing v/a of sarcoplasmic reticulum.
出处
《中国应用生理学杂志》
CAS
CSCD
2013年第2期162-165,共4页
Chinese Journal of Applied Physiology
基金
2009年山东省高等学校优秀青年教师国内访问学者项目(2009GN003)
2011滨州医学院大学生科技创新项目(BY2011DKCX12)
关键词
槲皮素
小肠平滑肌
钙离子
一氧化氮
quercetin
small intestines smooth muscle
calcium
nitrogen monoxidum
