摘要
中国沙棘（HippophaerhamnoidesL．subsp．chinensisRousi），又名醋柳，系胡颓子科（Elaeagnaceae），沙棘属（Hippophae），别名醋柳果、醋刺柳、酸刺子、黑刺、沙枣，大量分布于华北、东北、西北和西南，为传统藏药，干燥成熟的果实入药，具有止咳祛痰，消食化滞，活血化瘀等功效。现代研究证明沙棘果和沙棘叶中含有多种活性物质，并具有解重金属毒、放射保护、抗氧化应激、抗肿瘤、抗炎、抗溃疡、抗病毒和调节免疫等多种药理作用。
The enzyme-inhibitor model and the sugar tolerance mouse model were used to evaluate the relationship between the inhibition rate of enzyme activity and concentration of Hippophae rhamnoides L. subsp. chinensis Rousi polysaccharide （HRP）. The inhibitory patterns of enzyme and dose-dependent effects of HRP＇s effect on blood glucose using acarbose tablets as control were also examined. The mechanism underlying hypoglycemic effects of HRP was discussed. The results showed： in the enzyme-inhibitor model, the inhibitory activity of different concentrations of HRP （9.80, 19.60, 39.20, 78.40, 156.80 and 312.50 mg .L-1） on a-glucosaminidase （AG） inhibitory activity were 6.62%, 18.02%, 33.26%, 48.23%, 62.11%, 76.31% , 90.12%, ICs0 was 31.59 mg.L-1. The inhibitory rate of 25.00x103 mg-L-1 acarbose tablets was only 64.87%, and ICs0 was 10.75X03 mg.L 1. In the sugar tolerance mouse model, different doses of HRP （240, 480, 960 mg.kg-1） tended to decrease levels of blood glucose compared with control group （acarbose tablets 375 mg-kg-1） at 15, 30, 60 and 120 min. It＇s further confirmed that HRP is a kind of competitive inhibitor of AG activity. Its inhibition rate increases with the increase of concentration in normal mice, and it subsequently improves the sugar tolerance showing the effect of reducing blood sugar.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第4期604-608,共5页
Acta Pharmaceutica Sinica
基金
青海省自然科学基金资助项目(2012-Z-909)
关键词
沙棘
多糖
Α-葡萄糖苷酶
酶抑制剂
葡萄糖耐量
血糖
hippophae
polysaceharide
alpha-glucosidase
enzyme inhibitor
glucose tolerance
bloodglucose
