摘要
为了全面快速地阐明半夏泻心汤的化学组成及其归属,本文利用超高效液相色谱与串联四极杆飞行时间质谱仪联用技术(UPLC/Q-TOF-MSE)在相同实验条件下对半夏泻心汤及其组方各单味药的色谱图进行比对分析,归属了色谱峰来源,并根据高分辨质谱数据和对照品信息对色谱峰进行指认。从半夏泻心汤色谱图中共鉴定了74个色谱峰,主要成分包括黄酮类、三萜皂苷类、生物碱类、糖苷类等。本研究比较全面地阐明了半夏泻心汤的化学组成,为半夏泻心汤的药效物质基础研究和质量控制奠定了基础。
In order to clarify the chemical composition and source of Banxia Xiexin decoction quickly and comprehensively, whole and individual herbs of Banxia Xiexin decoction were analyzed by ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry （UPLC/Q-TOF-MSe）. Under identical experiment conditions, chromatography results were compared between experiment groups. Based on the Q-TOF-MSE analysis, 74 peaks were identified on line. The herbal sources of these peaks were assigned. The results implied that flavonoids, triterpenoid saponins, alkaloids and glycosides were the main components in effective part of Banxia Xiexin decoction. The method established is simple and rapid for elucidation the constituents of Banxia Xiexin decoction and the results could be used for the quality control of Banxia Xiexin decoction.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第4期526-531,共6页
Acta Pharmaceutica Sinica
基金
“重大新药创制”科技重大专项资助项目(2012ZX09301-002)
国家自然科学基金资助项目(81274054)
