摘要
用组织学的方法研究了50μg/L 17β-雌二醇(E2)持续50 d暴露对剑尾鱼(Xiphophorus helleri)精巢和肝组织结构变化的影响。结果显示,剑尾鱼受E2暴露后,精巢内的精小囊数量减少,精原细胞发育停滞,精子数量稀少甚至没有;肝细胞胞质内出现脂肪空泡和脂肪沉积等脂肪肝症状。结果表明,E2具强雌激素效应,对剑尾鱼精巢和肝组织的损伤具有时间效应,随着暴露时间延长其损伤更严重,但都在其耐受范围之内,未造成鱼体的死亡。精巢组织结构损伤可作为检测水环境雌激素污染生物标记。
The histological structural changes in the testis and liver tissues of the swordtail fish （Xiphophorus helleri） exposed to 50 μg/L 17β-estradiol （E2） for 50 days were investiged. The results showed that, after exposure to E2 , the number of spermatogonia was reduced, the growth and development of spermatogonia were stagnated, and the number of sperm was decreased. The liver cells exhibited fat vacuoles and fat deposition, indicative of fatty liver symptoms. The results indicated that E2 had strong estrogenic effects. The extent of testis and liver tissue damage changed with E2 exposure time. The longer the exposure, the more serious the damage was. But the damage was still within the tolerance of the fish, without causing its death. Therefore, E2 exposure damage to the testis structure can be used as a histological biomarker for detection of environmental estrogens.
出处
《动物学杂志》
CAS
CSCD
北大核心
2013年第2期174-181,共8页
Chinese Journal of Zoology
基金
广东省科技计划项目(No.2012B030800006)
关键词
17Β-雌二醇
精巢
肝
剑尾鱼
17β-estradiol
Testis
Liver
Xiphophorus helleri
