摘要
目的通过分析各萜类合成酶的表达,预测各种环境因素和逆境处理对白木香萜类合成的影响。方法对二年生白木香植株和愈伤组织分别进行多种逆境处理,采用荧光定量PCR分析组织中萜类合成酶基因的表达模式。结果伤害和火烙均可诱导茎干萜类合成酶转录水平的表达,火烙法诱导效果更明显;低温抑制各类萜类合成酶的转录;愈伤组织的处理中,茉莉酸甲酯(MeJA)对萜类合成酶转录的诱导效果最显著。结论伤害和火烙均可诱导茎干中萜类的合成,但火烙效果更好,低温不利于白木香结香,多种处理均可诱导愈伤组织萜类合成,其中MeJA诱导效果最佳。
Objective To analyze the expression of terpene synthases ofAquilaria sinensis, an endangered south medicine, and to predict the influences of the environmental factors and stresses on the synthesis of terpene in A. sinensis. Methods Two-year old seedlings and calli were treated by different stresses. The gene expression patterns of terpene synthases were analyzed by real-time qPCR. Results Wounding and cauterizing could induce the transcriptional expression ofterpene synthases in the stems, and the effect of cauterizing was more significant. Low temperature inhibited the transcription of terpene synthases. In calli, methyl jasmonate （MeJA） treatment had the most effective induction. Conclusion Both wounding and cauterizing could induce the synthesis ofterpenes in stems, while the cauterizing treatment might have better effect. Low temperature has negative influence on agarwood formation. For calli, different treatments could induce the terpene synthesis, while MeJA has the best efficacy.
出处
《中草药》
CAS
CSCD
北大核心
2013年第6期749-754,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81001607)
关键词
白木香
萜类
萜类合成酶
逆境处理
荧光定量PCR
Aquilaria sinensis （Lour.） Spreng,
terpene
terpene synthase
stress treatment
real-time qPCR
